Inhibition of MAP kinase kinase (MEK) blocks endothelial PGI(2) release but has no effect on von Willebrand Factor secretion or E-selectin expression

被引：42

作者：

WheelerJones, CPD

May, MJ

Houliston, RA

Pearson, JD

机构：

[1] Vascular Biology Research Centre, King's College London, Kensington, London W8 7AH, Campden Hill Road

来源：

FEBS LETTERS | 1996年 / 388卷 / 2-3期

关键词：

human endothelial cell; MAP kinase; thrombin; cytokine; prostacyclin; adhesion molecule;

D O I：

10.1016/0014-5793(96)00547-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the potential role of MAP kinase in the regulation of endothelial cell PGI(2) synthesis, VWF secretion and E-selectin expression using the specific MEK inhibitor PD98059. PD98059 dose-dependently attenuated the tyrosine phosphorylation and activation of p42(mapk) in response to thrombin or inflammatory cytokines. Inhibition of thrombin-induced p42(mapk) activation was paralleled by an inhibitory effect of PD98059 on thrombin-driven PGI(2) generation but not on vWF secretion or IL-1 alpha/TNF alpha-induced E-selectin expression. These results provide evidence for a key role for p42(mapk) in the acute regulation of PGI(2) synthesis in human endothelial cells and suggest that activation of the MAP kinase cascade is not obligatory for cytokine-stimulated E-selectin expression.

引用

页码：180 / 184

页数：5

共 27 条

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