Optically sectioned fluorescence lifetime imaging using a Nipkow disk microscope and a tunable ultrafast continuum excitation source

被引:35
作者
Grant, DM
Elson, DS
Schimpf, D
Dunsby, C
Requejo-Isidro, J
Auksorius, E
Munro, I
Neil, MAA
French, PMW
Nye, E
Stamp, G
Courtney, P
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, London SW7 2BW, England
[2] London Res Inst, Expt Pathol Lab, Lincolns Inn Fields Labs, London WC2A 3PX, England
[3] Univ London Imperial Coll Sci Technol & Med, Dept Investigat Sci, London SW7 2AZ, England
关键词
D O I
10.1364/OL.30.003353
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We demonstrate an optically sectioned fluorescence lifetime imaging microscope with a wide-field detector, using a convenient, continuously tunable (435-1150 nm) ultrafast source for fluorescence imaging applications that is derived from a visible supercontinuum generated in a microstructured fiber. (c) 2005 Optical Society of America.
引用
收藏
页码:3353 / 3355
页数:3
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