The salicylate trapping method: Is oxidation of salicylic acid solution oxygen and time dependent and metal catalysed?

被引：8

作者：

Gruber, M

Wiesner, G

Burger, R

Lindner, R

机构：

[1] Univ Regensburg, Dept Anaesthesiol, D-93053 Regensburg, Germany

[2] German Heart Ctr Munich, Inst Anasthesiol, D-80636 Munich, Germany

[3] Univ Regensburg, Dept Neurosurg, D-93053 Regensburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 831卷 / 1-2期

关键词：

salicylate trapping method; 2,5-DHBA; 2,3-DHBA; hyperoxia; oxygen; metal catalysis;

D O I：

10.1016/j.jchromb.2005.11.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For a microdialytic trapping method we systematically investigated changes in concentrations of 2,5-dihydroxy-benzoic acid (2,5-DHBA) and 2,3-dihydroxy-benzoic acid (2,3-DHBA) in freshly prepared solutions of salicylic acid (SA). The solvent was 0.9% saline exposed to different atmospheric concentrations of oxygen (0, 21, and 100%). The solutions were treated by freezing-thawing and an ultrasonic bath in presence and absence of aluminium foil. Without aluminium the concentrations of 2,5-DHBA and 2,3-DHBA kept constant over an observed period of 160 min on different levels from below 20 ng/ml to about 100 ng/ml. In presence of aluminium the concentrations increased to maximum 307 ng/ml after 160 min. Ultrasonic irradiation amplified this effect to maximum 341 ng/ml. HPLC/ECD processing and quantitative analysis of dihydroxy-benzoic acids (DHBAs) in microdialysis may be artificially influenced by varying oxygen environment and metal catalysis. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：320 / 323

页数：4

共 28 条

[11] INTRACRANIAL MICRODIALYSIS OF SALICYLIC-ACID TO DETECT HYDROXYL RADICAL GENERATION THROUGH DOPAMINE AUTOOXIDATION IN THE CAUDATE-NUCLEUS - EFFECTS OF MPP [J].