Dextromethorphan Inhibits Activations and Functions in Dendritic Cells

被引：20

作者：

Chen, Der-Yuan ^{[1
,2
,3
]}

Song, Pei-Shan ^{[1
]}

Hong, Jau-Shyong ^{[4
]}

Chu, Ching-Liang ^{[5
]}

Pan, I-Horng ^{[6
]}

Chen, Yi-Ming ^{[3
,7
]}

Lin, Ching-Hsiung ^{[8
,9
,10
]}

Lin, Sheng-Hao ^{[1
,8
]}

Lin, Chi-Chen ^{[1
,11
]}

机构：

[1] Natl Chung Hsing Univ, Inst Biomed Sci, Taichung 402, Taiwan

[2] Natl Yang Ming Univ, Fac Med, Taipei 112, Taiwan

[3] Taichung Vet Gen Hosp, Div Allergy Immunol & Rheumatol, Taichung 407, Taiwan

[4] NIEHS, Lab Toxicol & Pharmacol, Res Triangle Pk, NC 27709 USA

[5] Natl Taiwan Univ, Grad Inst Immunol, Coll Med, Taipei 100, Taiwan

[6] Ind Technol Res Inst, Biomed Technol & Device Res Labs, Hsinchu 300, Taiwan

[7] Natl Yang Ming Univ, Inst Clin Med, Taipei 112, Taiwan

[8] Changhua Christian Hosp, Div Chest Med, Dept Internal Med, Changhua 500, Taiwan

[9] Chang Jung Christian Univ, Dept Resp Care, Coll Hlth Sci, Tainan 711, Taiwan

[10] Chung Shan Med Univ, Sch Med, Taichung 402, Taiwan

[11] Taichung Vet Gen Hosp, Dept Med Res & Educ, Taichung 407, Taiwan

来源：

CLINICAL & DEVELOPMENTAL IMMUNOLOGY | 2013年

关键词：

NF-KAPPA-B; OXIDATIVE STRESS; TNF-ALPHA; MATURATION; DIFFERENTIATION; RESPONSES; LIPOPOLYSACCHARIDE; INVOLVEMENT; EXPRESSION; TOLERANCE;

D O I：

10.1155/2013/125643

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Dendritic cells (DCs) play an important role in connecting innate and adaptive immunity. Thus, DCs have been regarded as a major target for the development of immunomodulators. In this study, we examined the effect of dextromethorphan (DXM), a common cough suppressant with a high safety profile, on the activation and function of DCs. In the presence of DXM, the LPS-induced expression of the costimulatory molecules in murine bone marrow-derived dendritic cells (BMDCs) was significantly suppressed. In addition, DXM treatment reduced the production of reactive oxygen species (ROS), proinflammatory cytokines, and chemokines in maturing BMDCs that were activated by LPS. Therefore, DXM abrogated the ability of LPS-stimulated DCs to induce Ag-specific T-cell activation, as determined by their decreased proliferation and IFN-gamma secretion in mixed leukocyte cultures. Moreover, the inhibition of LPS-induced MAPK activation and NF-kappa B translocation may contribute to the suppressive effect of DXM on BMDCs. Remarkably, DXM decreased the LPS- induced surface expression of CD80, CD83, and HLA-DR and the secretion of IL-6 and IL-12 in human monocyte-derived dendritic cells (MDDCs). These findings provide a new insight into the impact of DXM treatment on DCs and suggest that DXM has the potential to be used in treating DC-related acute and chronic diseases.

引用

页数：11

共 59 条

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