Revisionist look at solvophobic driving forces in reversed-phase liquid chromatography .3. Comparison of the behavior of nonpolar and polar solutes

被引:53
作者
Carr, PW
Tan, LC
Park, JH
机构
[1] YEUNGNAM UNIV,DEPT CHEM,KYONGSAN 712749,SOUTH KOREA
[2] UNIV MINNESOTA,DEPT CHEM,MINNEAPOLIS,MN 55455
基金
美国国家科学基金会;
关键词
retention mechanisms; free energy of transfer; solvophobic driving forces; thermodynamic parameters;
D O I
10.1016/0021-9673(95)00907-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Recently we showed that in reversed-phase liquid chromatography (RPLC), solute methylene units are embedded in the bonded alkyl chains of the stationary phase. That is, the ''partition mechanism'', not the ''adsorption mechanism'', controls retention of methylene groups. We have also shown that the net interactions of the nonpolar groups of a solute with a nonpolar stationary phase contribute more to the overall free energy of transfer than does the solvophobic interaction in the mobile phase. The present work explores the behavior of several polar functional groups (Cl, OCH3, CHO, NO2, CN, and COOCH3) in RPLC. In contrast to the behavior of methylene groups, the net interactions of more polar functional groups with the mobile phase are larger than are their net interactions with the stationary phase. Further, the data indicate that the free energy of transfer of a polar functional group in bonded phase RPLC is quite different from the free energy of transfer between the mobile phase and a bulk nonpolar liquid used to emulate the bonded phase. This implies that either sorbed mobile phase significantly influences the retention properties of polar groups in the bonded phase, or that the solute functional groups reside at the interface between the mobile and stationary phases.
引用
收藏
页码:1 / 12
页数:12
相关论文
共 54 条
[51]   COMPARISON OF THE CHROMATOGRAPHIC RETENTION IN OCTADECYL-BONDED SILICA (METHANOL-WATER) SYSTEM WITH PARTITION-COEFFICIENT IN DODECANE (METHANOL-WATER) SYSTEM [J].
TSUKAHARA, S ;
SAITOH, K ;
SUZUKI, N .
ANALYTICAL SCIENCES, 1993, 9 (01) :71-76
[52]  
WEIMER RF, 1965, HYDROCARB PROCESS, V44, P237
[53]   COMPARISON OF STATIONARY PHASE FORMATION IN RP-FOR METHANOL-WATER SYSTEMS [J].
YONKER, CR ;
ZWIER, TA ;
BURKE, MF .
JOURNAL OF CHROMATOGRAPHY, 1982, 241 (02) :257-268
[54]   INVESTIGATION OF STATIONARY PHASE FORMATION FOR RP-18 USING VARIOUS ORGANIC MODIFIERS [J].
YONKER, CR ;
ZWIER, TA ;
BURKE, MF .
JOURNAL OF CHROMATOGRAPHY, 1982, 241 (02) :269-280