A recombinant vaccine against hydatidosis:: production of the antigen in Escherichia coli

被引:36
作者
Manderson, D
Dempster, R
Chisti, Y
机构
[1] Massey Univ, Inst Technol & Engn PN456, Palmerston North, New Zealand
[2] AgResearch, Wallaceville Anim Res Ctr, Upper Hutt, New Zealand
关键词
Echinococcus granulosus; Escherichia coli; hydatid; hydatidosis; recombinant vaccine; veterinary vaccine;
D O I
10.1007/s10295-005-0046-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A commercial process was developed for producing a recombinant vaccine against hydatidosts in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch Cultures of E. coli using Terrific broth in stirred bioreactors at 37 degrees C, pH 7.0, and a dissolved oxygen level of 30% of air saturation produced the highest volumetric concentrations of the final solubilized antigen. An exponential feeding strategy proved distinctly Superior to feeding based on pH-stat and DO-stat methods. The plasmid coding for the antigen was induced with isopropyl-beta-D-thlogalactopyranoside (IPTG) at 4 h after initiation of the culture. The minimum IPTG concentration for full induction was 0.1 mM.
引用
收藏
页码:173 / 182
页数:10
相关论文
共 49 条
[1]  
Åkesson M, 1999, BIOTECHNOL BIOENG, V64, P590, DOI 10.1002/(SICI)1097-0290(19990905)64:5<590::AID-BIT9>3.0.CO
[2]  
2-T
[3]   Avoiding acetate accumulation in Escherichia coli cultures using feedback control of glucose feeding [J].
Åkesson, M ;
Hagander, P ;
Axelsson, JP .
BIOTECHNOLOGY AND BIOENGINEERING, 2001, 73 (03) :223-230
[4]  
ATLAS JG, 1994, BERGEYS MANUAL DETER
[5]   Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E-coli [J].
Bhattacharya, SK ;
Dubey, AK .
ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (05) :355-360
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   Characteristics of a DO-controlled fed-batch culture of Escherichia coli [J].
Castan, A ;
Enfors, SO .
BIOPROCESS ENGINEERING, 2000, 22 (06) :509-515
[8]   Recombinant bioprocess optimization for heterologous protein production using two-stage, cyclic fed-batch culture [J].
Chang, CC ;
Ryu, DDY ;
Park, CS ;
Kim, JY ;
Ogrydziak, DM .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (05) :531-537
[9]   OPTIMIZATION FOR A RECOMBINANT ESCHERICHIA-COLI FED-BATCH FERMENTATION [J].
CHEN, Q ;
BENTLEY, WE ;
WEIGAND, WA .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1995, 51-2 :449-461
[10]  
Chisti Y, 1996, CHEM ENG RES DES, V74, P575