Simplified method for the determination of 25-hydroxy and 1 alpha,25-dihydroxy metabolites of vitamins D-2 and D-3 in human plasma - Application to nutritional studies

被引：6

作者：

Takeuchi, A

Ishida, Y

Sekimoto, H

Masuda, S

Okano, T

Nishiyama, S

Matsuda, I

Kobayashi, T

机构：

[1] KOBE PHARMACEUT UNIV,DEPT HYG SCI,HIGASHINADA KU,KOBE,HYOGO 658,JAPAN

[2] KUMAMOTO UNIV,SCH MED,DEPT PEDIAT,KUMAMOTO 860,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 691卷 / 02期

关键词：

vitamins; 25-hydroxyvitamin D-2; 1; alpha; 25-dihydroxyvitamin D-2; 25-hydroxyvitamin D-3; 25-dihydroxy-vitamin D-3;

D O I：

10.1016/S0378-4347(96)00443-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified method for the determination of 25-hydroxy and 1 alpha,25-dihydroxy metabolites of vitamins D-2 and D-3 in human plasma was developed. Plasma samples were deproteinizated and applied to a Bond Elut C18OH cartridge to separate 25-hydroxyvitamin D (25-OH-D) and 1 alpha,25-dihydroxyvitamin D [1,25(OH)(2)D] fractions. The 25-OH-D fraction was purified by a Bond Elut C-18 cartridge and 25-OH-D-2 and 25-OH-D-3 were assayed by HPLC using a Zorbax SIL column. The 1,25(OH)(2)D fraction obtained above was subsequently applied to HPLC using a Zorbax SIL column to separate 1,25(OH)(2)D-2 and 1,25(OH)(2)D-3 fractions which were determined by a radioreceptor assay (RRA) using calf thymus receptor. The method was applied to nutritional studies.

引用

页码：313 / 319

页数：7

共 20 条

[1] VITAMIN-D(3) AND CALCIUM TO PREVENT HIP-FRACTURES IN ELDERLY WOMEN [J].