The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

被引：294

作者：

Huggett, Jim F. ^{[1
,2
]}

Whale, Alexandra S. ^{[1
]}

De Spiegelaere, Ward ^{[3
]}

Nour, Afif Abdel ^{[4
]}

Bae, Young-Kyung ^{[5
]}

Benes, Vladimir ^{[6
]}

Burke, Daniel ^{[7
]}

Cleveland, Megan ^{[8
]}

Corbisier, Philippe ^{[9
]}

Devonshire, Alison S. ^{[1
]}

Dong, Lianhua ^{[10
]}

Drandi, Daniela ^{[11
]}

Foy, Carole A. ^{[1
]}

Garson, Jeremy A. ^{[12
]}

He, Hua-Jun ^{[8
]}

Hellemans, Jan ^{[13
]}

Kubista, Mikael ^{[14
,15
]}

Lievens, Antoon ^{[9
]}

Makrigiorgos, Mike G. ^{[16
]}

Milavec, Mojca ^{[17
]}

Mueller, Reinhold D. ^{[18
]}

Nolan, Tania ^{[19
,20
]}

O'Sullivan, Denise M. ^{[1
]}

Pfaffl, Michael W. ^{[21
]}

Roediger, Stefan ^{[22
,23
]}

Romsos, Erica L. ^{[8
]}

Shipley, Gregory L. ^{[24
]}

Taly, Valerie ^{[25
]}

Untergasser, Andreas ^{[6
,26
]}

Wittwer, Carl T. ^{[27
]}

Bustin, Stephen A. ^{[28
]}

Vandesompele, Jo ^{[3
,13
]}

机构：

[1] LGC, Natl Measurement Lab NML, Teddington, Middx, England

[2] Univ Surrey, Fac Hlth & Med Sci, Sch Biosci & Med, Guildford, Surrey, England

[3] Univ Ghent, Ghent, Belgium

[4] Holy Spirit Univ Kaslik, Sch Engn, Jounieh, Lebanon

[5] KRISS, Ctr Bioanal, Daejeon, South Korea

[6] EMBL Heidelberg, Genom Core Facil, Heidelberg, Germany

[7] Natl Measurement Inst, Lindfield, NSW, Australia

[8] NIST, Gaithersburg, MD 20899 USA

[9] European Commiss, Joint Res Ctr JRC, Geel, Belgium

[10] Natl Inst Metrol, Beijing, Peoples R China

[11] Univ Torino, Dept Mol Biotechnol & Hlth Sci, Turin, Italy

[12] UCL, Div Infect & Immun, Res Dept Infect, London, England

[13] Biogazelle, Zwijnaarde, Belgium

[14] TATAA Bioctr, Gothenburg, Sweden

[15] Czech Acad Sci, Inst Biotechnol, Gothenburg, Sweden

[16] Harvard Med Sch, Dana Farber Canc Inst, Boston, MA 02115 USA

[17] Natl Inst Biol, Dept Biotechnol & Syst Biol, Ljubljana, Slovenia

[18] RM Consulting, San Diego, CA USA

[19] Univ Manchester, Fac Med & Human Sci, Inst Populat Hlth, Manchester, Lancs, England

[20] Gene Team Ltd, Bardwell, Bury St Edmunds, England

[21] Tech Univ Munich, Sch Life Sci Weihenstephan, Anim Physiol & Immunol, Freising Weihenstephan, Germany

[22] Brandenburg Med Sch Theodor Fontane, Joint Fac Brandenburg Univ Technol Cottbus Senfte, Fac Hlth Sci, Berlin, Germany

[23] Univ Potsdam, Berlin, Germany

[24] Shipley Consulting LLC, Houston, TX USA

[25] Univ Paris, Sorbonne Univ, INSERM, Ctr Rech Cordeliers,Equipe Labellisee Ligue Natl, Paris, France

[26] Heidelberg Univ, Zentrum Mol Biol, Heidelberg, Germany

[27] Univ Utah, Dept Pathol, Salt Lake City, UT USA

[28] Anglia Ruskin Univ, Fac Hlth Educ Med & Social Care, Med Technol Res Ctr, Chelmsford, Essex, England

来源：

CLINICAL CHEMISTRY | 2020年 / 66卷 / 08期

基金：

欧盟地平线“2020”;

关键词：

POLYMERASE-CHAIN-REACTION; DNA COPY NUMBER; ABSOLUTE QUANTIFICATION; VOLUME VARIABILITY; DROPLET; QPCR; AMPLIFICATION; RESISTANCE; PLASMA; EGFR;

D O I：

10.1093/clinchem/hvaa125

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.

引用

页码：1012 / 1029

页数：18

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