Colicin V can be produced by lactic acid bacteria

被引：46

作者：

McCormick, JK

Klaenhammer, TR

Stiles, ME ^{[1
]}

机构：

[1] Univ Alberta, Dept Agr Food & Nutr Sci, Agr Forestry Ctr 4 10C, Edmonton, AB T6G 2P5, Canada

[2] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2P5, Canada

[3] N Carolina State Univ, Dept Food Sci, Raleigh, NC 27695 USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 29卷 / 01期

关键词：

D O I：

10.1046/j.1365-2672.1999.00571.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Grampositive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which recognize a double-glycine-type leader peptide on the immature colicin V bacteriocin. Replacement of the colicin V leader peptide by a signal peptide from the signal sequence-dependent bacteriocin divergicin A allowed expression of colicin V in lactic acid bacteria. This system may serve as a model for the heterologous expression of other small bacteriocins active against Gram-negative bacteria and other antibacterial peptides from lactic acid bacteria.

引用

页码：37 / 41

页数：5

共 26 条

[1] PLASMID-ASSOCIATED BACTERIOCIN PRODUCTION BY A STRAIN OF CARNOBACTERIUM-PISCICOLA FROM MEAT [J].