An effective method for bioconversion of delignified waste-cellulose fibers from the paper industry with a cellulase complex

被引：42

作者：

Nikolov, T ^{[1
]}

Bakalova, N ^{[1
]}

Petrova, S ^{[1
]}

Benadova, R ^{[1
]}

Spasov, S ^{[1
]}

Kolev, D ^{[1
]}

机构：

[1] Univ Sofia, Fac Biol, Dept Biochem, Sofia 1421, Bulgaria

来源：

BIORESOURCE TECHNOLOGY | 2000年 / 71卷 / 01期

关键词：

Trichoderma reesei M-7; cellulase enzyme complex; waste-cellulose fibers; acid pretreatment of waste fibers; enzymatic cellulose hydrolysis;

D O I：

10.1016/S0960-8524(99)00059-0

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

An effective method for production of glucose was developed using enzymatic hydrolysis of waste-cellulose fibers by the cellulase complex from Trichoderma reesei. However, these cellulosic materials are strongly resistant to direct enzymatic hydrolysis (the degree of degradation after 48 h of enzyme treatment did not exceed 14%)apparently due to the presence of various chemical substances used in the process of paper production. This adhesive "envelope" around the cellulose fibers was effectively pretreated with 0.25% H3PO4 and after that the free cellulose mass was extensively hydrolyzed by the cellulase complex (degree of degradation more than 80%). The HPLC analyses of the enzyme hydrolysates revealed glucose as the main component as well as some cellobiose and xylose. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1 / 4

页数：4

共 29 条

[1]

[Anonymous], [No title captured]

[2]

Bergmeyer H.U., 1983, Methods of Enzymatic Analysis, V2, P151, DOI [10.1007/BF00245352, DOI 10.1007/BF00245352]

[3]

DAVID C, 1985, APPL BIOCHEM BIOTECH, V11, P351, DOI 10.1007/BF02798669

[4] STRUCTURAL MODIFICATION OF LIGNOCELLULOSICS BY PRETREATMENTS TO ENHANCE ENZYMATIC-HYDROLYSIS [J].

GHARPURAY, MM ;

LEE, YH ;

FAN, LT .

BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (01) :157-172

[5] MEASUREMENT OF CELLULASE ACTIVITIES [J].

GHOSE, TK .

PURE AND APPLIED CHEMISTRY, 1987, 59 (02) :257-268

[6] ALKALINE PEROXIDE DELIGNIFICATION OF AGRICULTURAL RESIDUES TO ENHANCE ENZYMATIC SACCHARIFICATION [J].

GOULD, JM .

BIOTECHNOLOGY AND BIOENGINEERING, 1984, 26 (01) :46-52

[7]

Gregg DJ, 1996, BIOTECHNOL BIOENG, V51, P375, DOI [10.1002/(SICI)1097-0290(19960820)51:4&lt

[8]

375::AID-BIT1&gt

[9]

3.0.CO

[10]

2-F, 10.1002/(SICI)1097-0290(19960820)51:4<375::AID-BIT1>3.0.CO

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