Detection of human herpesvirus 7 DNA by loop-mediated isothermal amplification

被引：57

作者：

Yoshikawa, T ^{[1
]}

Ihira, M

Akimoto, S

Usui, C

Miyake, F

Suga, S

Enomoto, Y

Suzuki, R

Nishiyama, Y

Asano, Y

机构：

[1] Fujita Hlth Univ, Sch Med, Dept Pediat, Toyoake, Aichi 4701192, Japan

[2] Fujita Hlth Univ Coll, Dept Med Informat Technol, Toyoake, Aichi, Japan

[3] Nagoya Univ, Grad Sch Med, Dept Virol, Nagoya, Aichi, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 03期

关键词：

D O I：

10.1128/JCM.42.3.1348-1352.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The reliability of loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this study. Although a LAMP product was detected in HHV-7 DNA, neither HHV-6 nor human cytomegalovirus DNA produced a product. When agarose gel electrophoresis was used for the detection of LAMP products, the sensitivity of a 30-min HHV-7 LAMP reaction reached 250 copies/tube. The use of turbidity for the detection of the LAMP products gave a sensitivity of 500 and 250 copies/tube for 30- and 60-min reactions, respectively. Following these initial validation studies, clinical samples collected from two patients with primary HRV-7 infections were examined by HHV-7 LAMP. By use of agarose gel electrophoresis, HHV-7 LAMP products could be detected in acute-phase plasma samples but no LAMP product was detectable in convalescent-phase plasma samples from either patient. Since a turbidity assay is less sensitive than agarose gel electrophoresis, no HHV-7 LAMP product could be detected in plasma samples after a 30-min LAMP reaction. After a 60-min LAMP reaction, HHV-7 LAMP product could be detected in acute-phase plasma samples.

引用

页码：1348 / 1352

页数：5

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