Sialylation is essential for early development in mice

被引：288

作者：

Schwarzkopf, M

Knobeloch, KP

Rohde, E

Hinderlich, S

Wiechens, N

Lucka, L

Horak, I

Reutter, W

Horstkorte, R

机构：

[1] Free Univ Berlin, Fachbereich Humanmed, Inst Mol Biol & Biochem, D-14195 Berlin, Germany

[2] Free Univ Berlin, Forsch Inst Mol Pharmakol, D-12207 Berlin, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2002年 / 99卷 / 08期

关键词：

D O I：

10.1073/pnas.072066199

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sialic acids are widely expressed as terminal carbohydrates on glycoconjugates of eukaryotic cells. Sialylation is crucial for a variety of cellular functions, such as cell adhesion or signal recognition, and regulates the biological stability of glycoproteins. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetyImannosamine kinase (UDP-GlcNAc 2-epimerase), which catalyzes the first two steps of sialic acid biosynthesis in the cytosol. We report that inactivation of the UDP-GlcNAc 2-epimerase by gene targeting causes early embryonic lethality in mice, thereby emphasizing the fundamental role of this bifunctional enzyme and sialylation during development. The need of UDP-GlcNAc 2-epimerase for a defined sialylation process is exemplified with the polysialylation of the neural cell adhesion molecule in embryonic stem cells.

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页码：5267 / 5270

页数：4

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