Aluminum induces low phosphate adaptive responses and modulates primary and lateral root growth by differentially affecting auxin signaling in Arabidopsis seedlings

被引:30
作者
Francisco Ruiz-Herrera, Leon [1 ]
Lopez-Bucio, Jose [1 ]
机构
[1] Univ Michoacana, Inst Invest Quim Biol, Morelia 58030, Michoacan, Mexico
关键词
Aluminum; Phosphate deficiency; Arabidopsis thaliana; Auxin mutants; Root development; BOX PROTEIN TIR1; ORGANIC-ACIDS; PHOSPHORUS; RESISTANCE; INHIBITION; TOLERANCE; SENSITIVITY; ELONGATION; SPECIATION; EXUDATION;
D O I
10.1007/s11104-013-1722-0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The aims of this work were to investigate the aluminum (Al) and phosphate (P) interactions in the regulation of root system architecture of Arabidopsis thaliana seedlings and the contribution of auxin signaling in primary and lateral root growth in response to Al toxicity. Detailed analyses of root system architecture and cell division were performed in Arabidopsis WT seedlings and in low phosphorus insensitive mutants lpi1-3 and lpr1-1 lpr2-1 in response to Al. Expression studies of P-deficiency regulated phosphate transporter AtPT2 were also conducted. The role of auxin as a mediator of root morphogenetic changes by Al was evaluated by using the auxin-signaling mutants tir1, tir1 afb2 afb3, and arf7 arf19. Al inhibited primary root growth by affecting cell cycle progression and causing differentiation of cells in the root meristem. These effects were reduced in low phosphorus insensitive lpi1-3 and low phosphate resistant lpr1-1 lpr2-1 Arabidopsis mutants. Al also activated the expression of the low phosphate-induced P transporter AtPT2 in roots. Lateral root formation by Al decreased in tir1 afb2 afb3 while arf7 arf19 mutants were highly resistant to Al in both primary root inhibition and lateral root induction. Our results suggest that lateral root formation in response to Al toxicity and P deficiency may involve common signaling mechanisms, while a pathway involving ARF7 and ARF19 is important for primary root growth inhibition by Al.
引用
收藏
页码:593 / 609
页数:17
相关论文
共 37 条
[1]   Aluminium speciation and phytotoxicity in alkaline soils [J].
Brautigan, D. J. ;
Rengasamy, P. ;
Chittleborough, D. J. .
PLANT AND SOIL, 2012, 360 (1-2) :187-196
[2]   Spatio-temporal analysis of mitotic activity with a labile cyclin-GUS fusion protein [J].
Colón-Carmona, A ;
You, R ;
Haimovitch-Gal, T ;
Doerner, P .
PLANT JOURNAL, 1999, 20 (04) :503-508
[3]   The F-box protein TIR1 is an auxin receptor [J].
Dharmasiri, N ;
Dharmasiri, S ;
Estelle, M .
NATURE, 2005, 435 (7041) :441-445
[4]   Root cell patterning:: a primary target for aluminium toxicity in maize [J].
Doncheva, S ;
Amenós, M ;
Poschenrieder, C ;
Barceló, J .
JOURNAL OF EXPERIMENTAL BOTANY, 2005, 56 (414) :1213-1220
[5]   Aluminum resistance in two cultivars of Zea mays L.:: Root exudation of organic acids and influence of phosphorus nutrition [J].
Gaume, A ;
Mächler, F ;
Frossard, E .
PLANT AND SOIL, 2001, 234 (01) :73-81
[6]   Aluminium toxicity targets PIN2 in Arabidopsis root apices:: Effects on PIN2 endocytosis, vesicular recycling, and polar auxin transport [J].
Hong, Shen ;
Hou NingYan ;
Schlicht, Markus ;
Wan YingLang ;
Mancuso, Stefano ;
Baluska, Frantisek .
CHINESE SCIENCE BULLETIN, 2008, 53 (16) :2480-2487
[7]   Effect of aluminum on cytoplasmic Ca2+ homeostasis in root hairs of Arabidopsis thaliana (L.) [J].
Jones, DL ;
Gilroy, S ;
Larsen, PB ;
Howell, SH ;
Kochian, LV .
PLANTA, 1998, 206 (03) :378-387
[8]   Regulated expression of Arabidopsis phosphate transporters [J].
Karthikeyan, AS ;
Varadarajan, DK ;
Mukatira, UT ;
D'Urzo, MP ;
Damsz, B ;
Raghothama, KG .
PLANT PHYSIOLOGY, 2002, 130 (01) :221-233
[9]   The Arabidopsis F-box protein TIR1 is an auxin receptor [J].
Kepinski, S ;
Leyser, O .
NATURE, 2005, 435 (7041) :446-451
[10]   Reconsidering the rhizotoxicity of hydroxyl, sulphate, and fluoride complexes of aluminium [J].
Kinraide, TB .
JOURNAL OF EXPERIMENTAL BOTANY, 1997, 48 (310) :1115-1124