Excess production of interleukin-12 subunit p40 stimulated by the virulence plasmid of Salmonella enterica serovar Typhimurium in the early phase of infection in the mouse

被引：8

作者：

Chang, CC ^{[1
]}

Ou, JT ^{[1
]}

机构：

[1] Chang Gung Univ, Coll Med, Dept Microbiol & Immunol, Tao Yuan 333, Taiwan

来源：

MICROBIAL PATHOGENESIS | 2002年 / 32卷 / 01期

关键词：

Typhimurium; virulence plasmid; plasmidless; IL-12; IL-12p40; IL-12p35; mRNA; Tn5 insertion mutation; partial diploid;

D O I：

10.1006/mpat.2001.0476

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The production of interleukin-12 (IL-12) and its subunits in response to Salmonella enterica serovar Typhimurium infection in the BALB/c mouse was examined. Unlike wild-type Typhimurium, a plasmidless strain, isolated by curing of the virulence plasmid (pSTV), did not stimulate excess IL-12p40 production. When a Tn5 tagged pSTV was transferred back to the plasmidless strain, the ability to stimulate IL-12p40 production was restored. However, a strain harbouring another Tn50pSTV failed to stimulate excess IL-12p40 production. This Tn5 insertion area, located on fragment H3 of pSTV, was designated spf (stimulation of protein forty). The ability to stimulate IL-12p40 production was restored in a partial diploid that carried a wild-type fragment covering the spf site. There is one known gene, repA, a locus, rsk, and two putative ORFs, in the vicinity of the Tn5 insertion site; however, these are not spf. The precise location of the spf locus is still unknown. (C) 2002 Academic Press.

引用

页码：15 / 25

页数：11

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