Effect of acute heat shock on gene expression by human peripheral blood mononuclear cells

被引:94
作者
Sonna, LA
Gaffin, SL
Pratt, RE
Cullivan, ML
Angel, KC
Lilly, CM
机构
[1] USA, Environm Med Res Inst, Thermal & Mt Med Div, Natick, MA 01760 USA
[2] Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Pulm & Crit Care Med, Boston, MA 02115 USA
[3] Harvard Univ, Brigham & Womens Hosp, Sch Med, Partners Gene Array Technol Ctr, Boston, MA 02115 USA
关键词
apoptosis; gene chip array technology; cell stress response;
D O I
10.1152/japplphysiol.01002.2001
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We studied the effect of heat shock on gene expression by normal human cells. Peripheral blood mononuclear cells (PBMCs) were obtained from healthy adults. Paired samples from each subject were subjected to either 20 min of heat shock (43degreesC) or control (37degreesC) conditions and then returned to 37degreesC. RNA was isolated 160 min later, and five representative samples were analyzed on Affymetrix gene chip arrays containing similar to12,600 probes. A biologically meaningful effect was defined as a statistically significant, twofold or greater difference in expression of sequences that were detected in all five experiments under control (downregulated sequences) or heat shock (upregulated sequences) conditions. Changes occurred in 395 sequences (227 increased by heat shock, 168 decreased), representing 353 Unigene numbers, in every functional category previously implicated in the heat shock response. By RT-PCR, we confirmed the findings for one upregulated sequence (Rad, a G protein) and one downregulated sequence (osteopontin, a cytokine). We conclude that heat shock causes extensive gene expression changes in PBMCs, affecting all functional categories of the heat shock response.
引用
收藏
页码:2208 / 2220
页数:13
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