Toxoplasma gondii-derived heat shock protein 70 stimulates maturation of murine bone marrow-derived dendritic cells via Toll-like receptor 4

被引:52
作者
Aosai, F
Pena, MSR
Mun, HS
Fang, H
Mitsunaga, T
Norose, K
Kang, HK
Bae, YS
Yano, A
机构
[1] Chiba Univ, Grad Sch Med, Dept Infect & Host Def, Chuo Ku, Chiba 2608670, Japan
[2] Dong A Univ, Med Res Ctr Canc Mol Therapy, Busan 602714, South Korea
关键词
D O I
10.1379/CSC-138R.1
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Toxoplasma gondii-derived heat shock protein 70 (T.g.HSP70) induced maturation of bone marrow-derived dendritic cells (DCs) of wild-type (WT) C57BL/6 mice as evidenced by an increase in surface expression of MHC class I and II molecules and costimulatory molecules such as CD40, CD80, and CD86. Functionally, decreased phagocytic ability and increased alloreactive T cell stimulatory ability were observed in T.g.HSP70-stimulated DCs. These phenotypic and functional changes of T.g.HSP70-stimulated DCs were demonstrated in Toll-like receptor (TLR) 2- and myeloid differentiation factor 88 (MyD88)-deficient but not TLR4-deficient C57BL/6 mice. DCs from WT and TLR2-deficient but not TLR4-deficient mice produced IL-12 after T.g.HSP70 stimulation. T.g.HSP70-stimulated DCs from WT, TLR2-deficient, and MyD88-deficient, but not TLR4-deficient mice expressed IFN-beta mRNA. Thus, T.g.HSP70 stimulates murine DC maturation via TLR4 through the MyD88-independent signal transduction cascade.
引用
收藏
页码:13 / 22
页数:10
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