Recombinant glucocorticoid induced tumor necrosis factor receptor (rGITR) induces NOS in murine macrophage

被引：47

作者：

Shin, HH

Lee, MH

Kim, SG

Lee, YH

Kwon, BS

Choi, HS ^{[1
]}

机构：

[1] Univ Ulsan, Dept Biol Sci, Ulsan 680749, South Korea

[2] Univ Ulsan, Immunomodulat Res Ctr, Ulsan 680749, South Korea

来源：

FEBS LETTERS | 2002年 / 514卷 / 2-3期

关键词：

glucocorticoid induced tumor necrosis factor; receptor; inducible nitric oxide synthase; macrophage; nitric oxide;

D O I：

10.1016/S0014-5793(02)02379-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucocorticoid induced tumor necrosis factor receptor (GITR) is a new member of the tumor necrosis factor-nerve growth factor receptor superfamily of which the function has not been well studied. The extracellular domain of GITR was produced in Escherichia coli and purified as a single band of predicted M-r of 18.0 kDa. GITR and GITR ligand were expressed constitutively on the surface of Raw 264.7 macrophage cell line and murine peritoneal macrophages. An extracellular domain of GITR can activate murine macrophages to express inducible nitric oxide synthase and to generate nitric oxide in a dose- and time-dependent manner. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：275 / 280

页数：6

共 29 条

[1] 4-1BB and Ox40 are members of a tumor necrosis factor (TNF)-nerve growth factor receptor subfamily that bind TNF receptor-associated factors and activate nuclear factor κB [J].