CD4 T cells are the only lymphocytes needed to protect mice against rotavirus shedding after intranasal immunization with a chimeric VP6 protein and the adjuvant LT(R192G)

被引:83
作者
McNeal, MM
VanCott, JL
Choi, AHC
Basu, M
Flint, JA
Stone, SC
Clements, JD
Ward, RL
机构
[1] Childrens Hosp, Med Ctr, Div Infect Dis, Cincinnati, OH 45229 USA
[2] Tulane Univ, Med Ctr, Dept Microbiol & Immunol, New Orleans, LA 70112 USA
关键词
D O I
10.1128/JVI.76.2.560-568.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Intranasal immunization of mice with a chimeric VP6 protein and the mucosal adjuvant Escherichia coli heat labile toxin LT(R192G) induces nearly complete protection against marine rotavirus (strain EDIM [epizootic diarrhea of infant mice virus]) shedding for at least I year. The aim of this study was to identify the protective lymphocytes elicited by this new vaccine candidate. Immunization of mouse strains lacking one or more lymphocyte populations revealed that protection was dependent on alpha beta T cells but mice lacking gamma delta T cells and B cells remained fully protected. Furthermore, depletion of CD8 T cells in immunized B-cell-deficient mice before challenge resulted in no loss of protection, while depletion of CD4 T cells caused complete loss of protection. Therefore, alpha beta CD4 T cells appeared to be the only lymphocytes required for protection. As confirmation, purified splenic T cells from immunized mice were intraperitoneally injected into Rag-2 mice chronically infected with EDIM. Transfer of 2 x 10(6) CD8 T cells had no effect on shedding, while transfer of 2 x 10(5) CD4 T cells fully resolved shedding in 7 days. Interestingly, transfer of naive splenic CD4 T cells also resolved shedding but more time and cells were required. Together, these results establish CD4 T cells as effectors of protection against rotavirus after intranasal immunization of mice with VP6 and LT(R192G).
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页码:560 / 568
页数:9
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