Adenovirus vector-mediated expression of TMEM166 inhibits human cancer cell growth by autophagy and apoptosis in vitro and in vivo

被引：42

作者：

Chang, Ying ^{[1
,2
]}

Li, Yanjun ^{[1
,2
]}

Hu, Jia ^{[1
,2
]}

Guo, Jinhai ^{[3
]}

Xu, Dong ^{[1
,2
]}

Xie, Hong ^{[1
]}

Lv, Xiaodong ^{[1
]}

Shi, Taiping ^{[3
]}

Chen, Yingyu ^{[1
,2
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, Minist Hlth, Key Lab Med Immunol, Beijing 100191, Peoples R China

[2] Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China

[3] Chinese Natl Human Genome Ctr, Beijing, Peoples R China

来源：

CANCER LETTERS | 2013年 / 328卷 / 01期

基金：

中国国家自然科学基金;

关键词：

TMEM166; Adenovirus vector; Autophagy; Apoptosis; Anti-tumor activity; TUMOR-SUPPRESSOR; PROTEIN; BECLIN-1; DEATH; GENE; MACROAUTOPHAGY; TUMORIGENESIS; THERAPY; COMPLEX; KINASE;

D O I：

10.1016/j.canlet.2012.08.032

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

TMEM166 is a novel programmed cell death-related molecule. In this report, we constructed a recombinant adenovirus 5-TMEM166 vector (Ad5-TMEM166) and evaluated its expression and anti-tumor activities in vitro and in vivo. Cell viability analysis revealed that the adenovirus-mediated increase of TMEM166 inhibited tumor cell growth in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy (via inhibition of mTOR and activation of p70S6K) and apoptosis (via caspase-3 activation), both of which contributed to cell death and suppression of tumorigenicity. Our data indicated that Ad5-TMEM166 may be a novel gene therapy candidate for cancer. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：126 / 134

页数：9

共 34 条

[1]

The tumor suppressor PTEN positively regulates macroautophagy by inhibiting the phosphatidylinositol 3-kinase/protein kinase B pathway [J].