A mammalian RNA editing enzyme

被引：455

作者：

Melcher, T ^{[1
]}

Maas, S ^{[1
]}

Herb, A ^{[1
]}

Sprengel, R ^{[1
]}

Seeburg, PH ^{[1
]}

Higuchi, M ^{[1
]}

机构：

[1] UNIV HEIDELBERG, ZMBH, CTR MOLEC BIOL, MOLEC NEUROENDOCRINOL LAB, D-69120 HEIDELBERG, GERMANY

来源：

NATURE | 1996年 / 379卷 / 6564期

关键词：

D O I：

10.1038/379460a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

EDITING of RNA(1) by site-selective adcnosine deamination alters codons in brain-expressed pre-messenger RNAs for glutamate receptor (GluR) subunits(2-4) including a codon for a channel determinant (Q/R site) in GluR-B, which controls the Ca2+ permeability of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors(5,6). Editing of GluR pre-mRNAs requires a double-stranded RNA (dsRNA) structure formed by esonic and intronic sequences(4,7) and is catalysed by an unknown dsRNA adenosine deaminase. Here we report the cloning of complementary DNA for RED1, a dsRNA adenosine deaminase expressed in brain and peripheral tissues that efficiently edits the Q/R site in GluR-B pre-mRNA in vitro. This site is poorly edited by DRADA, which is distantly sequence related to RED1. Both deaminases edit the R/G site in GluR-B pre-mRNA, indicating that members of an emerging gene family catalyse adenosine deamination in nuclear transcripts with distinct but overlapping substrate specificities.

引用

页码：460 / 464

页数：5

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