A cellular ELISA to screen lectin-like compounds for cancer cell binding

被引：5

作者：

Chang, CP

Cheng, WC

Lei, HY ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Coll Med, Dept Microbiol & Immunol, Tainan, Taiwan

[2] Natl Cheng Kung Univ, Coll Med, Dept Basic Med Sci, Tainan, Taiwan

来源：

LETTERS IN DRUG DESIGN & DISCOVERY | 2005年 / 2卷 / 03期

关键词：

lectin; ELISA; apoptosis; mitogenic;

D O I：

10.2174/1570180053765228

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Lectins are carbohydrate-binding proteins that can modulate cellular activity through cell membrane glycoproteins. A cell-based binding was set up to screen the lectin-like substances in natural products. Lectins, after binding to tumor cells, are detected with a biotinylated carbohydrate-polyacrylamide complex by ELISA. Sugar specificity can also be determined within the assay.

引用

页码：182 / 188

页数：7

共 15 条

[1]

Abdullaev Fikrat I., 1997, Natural Toxins, V5, P157, DOI 10.1002/19970504NT6

[2] A lectin histochemistry comparative study in human normal prostate, benign prostatic hyperplasia, and prostatic carcinoma [J].