Rapid and Efficient Directed Differentiation of Human Pluripotent Stem Cells Into Retinal Pigmented Epithelium

被引：194

作者：

Buchholz, David E. ^{[1
,2
,3
]}

Pennington, Britney O. ^{[1
,2
,3
]}

Croze, Roxanne H. ^{[1
,2
,3
]}

Hinman, Cassidy R. ^{[1
,2
]}

Coffey, Peter J. ^{[1
,2
,4
]}

Clegg, Dennis O. ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif Santa Barbara, Ctr Stem Cell Biol & Engn, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Neurosci Res Inst, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Ctr Study Macular Degenerat, Santa Barbara, CA 93106 USA

来源：

STEM CELLS TRANSLATIONAL MEDICINE | 2013年 / 2卷 / 05期

关键词：

Cellular therapy; Differentiation; Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Retinal pigmented epithelium; ROCK INHIBITOR; MACULAR TRANSLOCATION; GROWTH-FACTOR; TRANSCRIPTION; DEGENERATION; INDUCTION; PATHWAYS; EYE;

D O I：

10.5966/sctm.2012-0163

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Controlling the differentiation of human pluripotent stem cells is the goal of many laboratories, both to study normal human development and to generate cells for transplantation. One important cell type under investigation is the retinal pigmented epithelium (RPE). Age-related macular degeneration (AMD), the leading cause of blindness in the Western world, is caused by dysfunction and death of the RPE. Currently, RPE derived from human embryonic stem cells are in clinical trials for the treatment of AMD. Although protocols to generate RPE from human pluripotent stem cells have become more efficient since the first report in 2004, they are still time-consuming and relatively inefficient. We have found that the addition of defined factors at specific times leads to conversion of approximately 80% of the cells to an RPE phenotype in only 14 days. This protocol should be useful for rapidly generating RPE for transplantation as well as for studying RPE development in vitro. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:384-393

引用

页码：384 / 393

页数：10

共 39 条

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