Development of a microconductometric biosniffer for detection of trimethylamine

被引：7

作者：

Fillit, Chrystelle ^{[1
]}

Jaffrezic-Renault, Nicole ^{[1
]}

Bessueille, Francois ^{[1
]}

Leonard, Didier ^{[1
]}

Mitsubayashi, Kohji ^{[2
]}

Tardy, Jacques ^{[3
]}

机构：

[1] Univ Lyon 1, CNRS, Sci Analyt Lab, UMR 5180, F-69622 Villeurbanne, France

[2] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Dept Biomed Devices & Instrumentat, Chiyoda Ku, Tokyo 1010062, Japan

[3] Ecole Cent Lyon, INL, CNRS, UMR 5270, F-69134 Ecully, France

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2008年 / 28卷 / 5-6期

关键词：

conductometric biosensor; trimethylamine; flavin-containing monooxygenase 3;

D O I：

10.1016/j.msec.2007.10.020

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The paper describes the development of a conductometric biosensor for detecting trimethylamine (TMA) which is a good parameter for estimating fish freshness. The biosensor consists of two parts: a microconductometric transducer and a structured enzymatic membrane using a flavin-containing monooxygenase 3 (FMO3) which is known for its sensitivity and selectivity for TMA. The process parameters for the fabrication of the biosniffer and various experimental variables such as pH, time of exposure to glutaraldehyde vapour were investigated with regard to their influence on sensitivity and limit of detection. A low detection limit of 2 mu g/mL (ppm) and a linear range up to 40 mu g/mL (ppm) were obtained. 90% response was reached in less than 10 min. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：781 / 786

页数：6

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