Application of enzyme field-effect transistors for determination of glucose concentrations in blood serum

被引:60
作者
Dzyadevich, SV
Korpan, YI
Arkhipova, VN
Alesina, MY
Martelet, C
El'Skaya, AV
Soldatkin, AP
机构
[1] Ukrainian Acad Sci, Inst Mol Biol & Genet, UA-252143 Kiev, Ukraine
[2] Chernobyl Sci & Tech Ctr Int Res, UA-255620 Chernobyl, Ukraine
[3] Ecole Cent Lyon, IfoS, UMR 5621, F-69131 Ecully, France
关键词
ENFET; glucose; blood serum; Nafion;
D O I
10.1016/S0956-5663(99)00007-X
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Glucose-sensitive enzyme held effect transistors (ENFETs) modified by an additional Naflon membrane have been developed and used for diluted blood samples analysis. The ENFET was used in the linear portion of the calibration curve up to 1.5 mM glucose in a model solution, which corresponds with up to 60 mM glucose in the undiluted samples (dilution 1:40). The high linearity of the Grans curve (factor of linearity is 1.03) obtained by the method of standard additions indicates the high precision of analysis. Glucose concentrations in different blood serum samples determined by ENFETs were compared with those measured by the commercial analyzer 'Eksan-G' and colorimetric method ('Diagluc' enzymatic kit), and good correlation between these methods was revealed. The high reproducibility and operational stability of the biosensor developed were demonstrated. (C) 1999 Elsevier Science S.A. All rights reserved.
引用
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页码:283 / 287
页数:5
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