Spin-on end-functional diblock copolymers for quantitative DNA immobilization

被引：24

作者：

Chen, Lu ^{[1
]}

Rengifo, Henan R. ^{[1
]}

Grigoras, Cristian ^{[1
]}

Li, Xiaoxu ^{[1
,2
]}

Li, Zengmin ^{[1
,2
]}

Ju, Jingyue ^{[1
,2
]}

Koberstein, Jeffrey T. ^{[1
]}

机构：

[1] Columbia Univ, Dept Chem Engn, New York, NY 10027 USA

[2] Columbia Univ Coll Phys & Surg, Columbia Genome Ctr, New York, NY 10032 USA

来源：

BIOMACROMOLECULES | 2008年 / 9卷 / 09期

关键词：

D O I：

10.1021/bm800258g

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate a simple means to covalently bond DNA to both hard (i.e., glass and silicon wafers) and soft (i.e., polymeric) substrates that provides quantitative and precise control of the DNA areal density. The approach is based on spin coating an alkyne-end-functional diblock copolymer, alpha-alkyne-omega-Br-poly(tBA-b-MMA), that self-assembles on both types of substrates as an ordered monolayer and thereby directs alkyne groups to the surface. Azido-functionalized DNA is covalently linked to the alkyne functionalized substrates by means of a "click" reaction between azide and alkyne groups. The density of immobilized DNA can be quantitatively controlled by varying the parameters used for spin-coating the copolymer film, that is, solution concentration and rotational speed, or by varying the copolymer molecular weight. We find the yield of the DNA coupling reaction to be dependent on the nature of the polymer underlying the reactive alkyne functional groups, being higher for more hydrophilic polymers.

引用

页码：2345 / 2352

页数：8

共 85 条

[41] Genomics, gene expression and DNA arrays [J].