Oxygen release from arterioles with normal flow and no-flow conditions

被引:15
作者
Cabrales, P
Tsai, AG
Johnson, PC
Intaglietta, M
机构
[1] Univ Calif San Diego, La Jolla Bioengn Inst, La Jolla, CA 92037 USA
[2] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92037 USA
关键词
oxygen consumption; occlusion; wall shear stress; arteriolar oxygen exit;
D O I
10.1152/japplphysiol.00762.2005
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The rate of oxygen release from arterioles (similar to 55 mu m diameter) was measured in the hamster window chamber model during flow and no-flow conditions. Flow was stopped by microvascular transcutaneous occlusion using a glass pipette held by a manipulator. The reduction of the intra- arteriolar oxygen tension (P-O2) was measured by the phosphorescence quenching of preinfused Pd-porphyrin, 100 mu m downstream from the occlusion. Oxygen release from arterioles was found to be 53% greater during flow than no-flow conditions (2.6 vs. 1.7 x 10(-5) ml O-2 (.) cm(-2) (.) s(-1), P < 0.05). Acute hemodilution with dextran 70 was used to reduce vessel oxygen content, significantly increase wall shear stress (14%, P < 0.05), reduce Hct to 28.4% (SD 1.0) [vs. 48.8% (SD 1.8) at baseline], lower oxygen supply by the arterioles (10%, P < 0.05), and increase oxygen release from the arterioles (39%, P < 0.05). Hemodilution also increased microcirculation oxygen extraction (33% greater than nonhemodilution, P < 0.05) and oxygen consumption by the vessel wall, as shown by an increase in vessel wall oxygen gradient [difference in P-O2 between the blood and the tissue side of the arteriolar wall, nonhemodiluted 16.2 Torr (SD 1.0) vs. hemodiluted 18.3 Torr (SD 1.4), P < 0.05]. Oxygen released by the arterioles during flow vs. nonflow was increased significantly after hemodilution (3.6 vs. 1.8 x 10(-5) ml O-2 (.) cm(-2) (.) s(-1), P < 0.05). The oxygen cost induced by wall shear stress, suggested by our findings, may be > 15% of the total oxygen delivery to tissues by arterioles during flow in this preparation.
引用
收藏
页码:1569 / 1576
页数:8
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