Monitoring nucleic acids using molecular beacons

被引：48

作者：

Yang, CYJ

Medley, CD

Tan, WH ^{[1
]}

机构：

[1] Univ Florida, Dept Chem, Ctr Res BioNano Interface, Gainesville, FL 32611 USA

[2] Univ Florida, Shands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA

[3] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA

来源：

CURRENT PHARMACEUTICAL BIOTECHNOLOGY | 2005年 / 6卷 / 06期

关键词：

molecular beacons; RNA; DNA; locked nucleic acids; signal amplification; fluorescence resonance energy transfer; single living cells;

D O I：

10.2174/138920105775159322

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to observe the dynamic of RNA in single living cells offers many exciting opportunities in biology and medicine. In the last few years, molecular beacons (MBs) have shown great potential in monitoring RNA synthesis, transportation, and localization with good sensitivity and selectivity. A hairpin structure probe, MB is a dual-labeled single stranded oligonucleotide that only fluoresces in the presence of target sequences. In this paper, the basic principle and design of MB will be described. The application of MB for RNA imaging in living cells will be reviewed. The limitations of MB for in vivo application will be identified. In the last section of the article, the efforts on designing better MBs for highly sensitive and selective RNA imaging will be discussed.

引用

页码：445 / 452

页数：8

共 66 条

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