Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen

被引：66

作者：

Liu, Tan ^{[1
]}

Wei, Qing ^{[1
]}

Wang, Jing ^{[1
]}

Jiao, Shuliang ^{[2
]}

Zhang, Hao F. ^{[1
]}

机构：

[1] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA

[2] Univ So Calif, Dept Ophthalmol, Los Angeles, CA 90033 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 05期

关键词：

RETINAL OXIMETRY; HIGH-RESOLUTION; DEGENERATION; RETINOPATHY; VESSELS; FMRI;

D O I：

10.1364/BOE.2.001359

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We proposed to measure the metabolic rate of oxygen (MRO2) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO2 by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO(2)) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO2 were further calculated. The measured blood flow rates in the two vessels agreed with each other. (C) 2011 Optical Society of America

引用

页码：1359 / 1365

页数：7

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