Fusion Partner Toolchest for the Stabilization and Crystallization of G Protein-Coupled Receptors

被引：328

作者：

Chun, Eugene ^{[1
]}

Thompson, Aaron A. ^{[1
]}

Liu, Wei ^{[1
]}

Roth, Christopher B. ^{[2
]}

Griffith, Mark T. ^{[2
]}

Katritch, Vsevolod ^{[1
]}

Kunken, Joshua ^{[1
]}

Xu, Fei ^{[1
]}

Cherezov, Vadim ^{[1
]}

Hanson, Michael A. ^{[2
]}

Stevens, Raymond C. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[2] Receptos, San Diego, CA 92121 USA

来源：

STRUCTURE | 2012年 / 20卷 / 06期

关键词：

CRYSTAL-STRUCTURE; OPIOID RECEPTOR; LIPIDIC MESOPHASES; MEMBRANE-PROTEINS; LACTOSE PERMEASE; ESCHERICHIA-COLI; COMPLEX; CRYSTALLOGRAPHY; ANTAGONIST; ANTIBODY;

D O I：

10.1016/j.str.2012.04.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural studies of human G protein-coupled receptors (GPCRs) have recently been accelerated through the use of a fusion partner that was inserted into the third intracellular loop. Using chimeras of the human beta(2)-adrenergic and human A(2A) adenosine receptors, we present the methodology and data for the initial selection of an expanded set of fusion partners for crystallizing GPCRs. In particular, use of the thermostabilized apocytochrome b(562)RIL as a fusion partner displays certain advantages over previously utilized fusion proteins, resulting in a significant improvement in stability and structure of GPCR-fusion constructs.

引用

页码：967 / 976

页数：10

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