Very high-pressure capillary liquid chromatography assisted by voltage

被引：12

作者：

Cintrón, JM ^{[1
]}

Colón, LA ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1106卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

liquid chromatography; voltage-assisted capillary liquid chromatography; capillary electrochromatogaphy; pressure assisted capillary electrochromatography;

D O I：

10.1016/j.chroma.2005.05.053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary liquid chromatography at moderately high pressures and capillary electrochromatography (CEC) have been combined to drive the mobile phase through capillary columns packed with small diameter particles. In a column packed with 1.5 mu m nonporous particles, linear velocities near 3 mm/s were observed when combining inlet pressures of 690 bar (10,000 psi) and an applied voltage of 25 kV. Optimum linear velocity for the column was achieved using a pressure-voltage combination of 350 bar (5000 psi) and 5 kV. Separation efficiencies at near optimum linear velocity agreed with those predicted by the van Deemter equation for liquid chromatography. Retention factors were observed to decrease under pressure-voltage combination as the voltage was increased; such a behavior has been attributed to Joule heating effects. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：131 / 138

页数：8

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