Cultured rat hippocampal neural progenitors generate spontaneously active neural networks

被引:52
作者
Mistry, SK
Keefer, EW
Cunningham, BA
Edelman, GM
Crossin, KL
机构
[1] Scripps Res Inst, Dept Neurobiol, La Jolla, CA 92037 USA
[2] Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
[3] Neurosci Inst, San Diego, CA 92121 USA
关键词
D O I
10.1073/pnas.022646599
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We previously demonstrated that the neural cell adhesion molecule (N-CAM) inhibited the proliferation of cultured rat hippocampal progenitor cells and increased the number of neurons generated. We demonstrate here that the continued presence of fibroblast growth factor 2 along with N-CAM or brain-derived neurotrophic factor over 12 days of culture greatly increased the number of both progenitors and neurons. These progenitor-derived neurons expressed neurotransmitters, neurotransmitter receptors, and synaptic proteins in vitro consistent with those expressed in the mature hippocampus. Progenitor cells cultured on microelectrode plates formed elaborate neural networks that exhibited spontaneously generated action potentials after 21 days. This activity was observed only in cultures grown in the presence of fibroblast growth factor 2 and either N-CAM or brain-derived neurotrophic factor. Analysis of neuronal activity after various pharmacological treatments indicated that the networks formed functional GABAergic and glutamatergic synapses. We conclude that mitogenic growth factors can synergize with N-CAM or neurotrophins to generate spontaneously active neural networks from neural progenitors.
引用
收藏
页码:1621 / 1626
页数:6
相关论文
共 54 条
  • [31] Glia-derived signals induce synapse formation in neurones of the rat central nervous system
    Nägler, K
    Mauch, DH
    Pfrieger, FW
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 2001, 533 (03): : 665 - 679
  • [32] The adult rat hippocampus contains primordial neural stem cells
    Palmer, TD
    Takahashi, J
    Gage, FH
    [J]. MOLECULAR AND CELLULAR NEUROSCIENCE, 1997, 8 (06) : 389 - 404
  • [33] Comparative anatomical distribution of serotonin 1A, 1D alpha and 2A receptor mRNAs in human brain postmortem
    Pasqualetti, M
    Nardi, I
    Ladinsky, H
    Marazziti, D
    Cassano, GB
    [J]. MOLECULAR BRAIN RESEARCH, 1996, 39 (1-2): : 223 - 233
  • [34] Synaptic efficacy enhanced by glial cells in vitro
    Pfrieger, FW
    Barres, BA
    [J]. SCIENCE, 1997, 277 (5332) : 1684 - 1687
  • [35] GENERATION OF NEURONS AND ASTROCYTES FROM ISOLATED CELLS OF THE ADULT MAMMALIAN CENTRAL-NERVOUS-SYSTEM
    REYNOLDS, BA
    WEISS, S
    [J]. SCIENCE, 1992, 255 (5052) : 1707 - 1710
  • [36] POTASSIUM AND CALCIUM-CHANNEL DEPENDENCE OF BURSTING IN CULTURED NEURONAL NETWORKS
    RHOADES, BK
    GROSS, GW
    [J]. BRAIN RESEARCH, 1994, 643 (1-2) : 310 - 318
  • [37] α5β1 integrin controls cyclin D1 expression by sustaining mitogen-activated protein kinase activity in growth factor-treated cells
    Roovers, K
    Davey, G
    Zhu, XY
    Bottazzi, ME
    Assoian, RK
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (10) : 3197 - 3204
  • [38] Sah DWY, 1997, J NEUROBIOL, V32, P95, DOI 10.1002/(SICI)1097-4695(199701)32:1<95::AID-NEU9>3.0.CO
  • [39] 2-9
  • [40] Shepherd G. M., 1988, NEUROBIOLOGY