A stable and sensitive voltammetric immunosensor based on a new non-enzymatic label

被引：27

作者：

Akter, Rashida ^{[1
]}

Rhee, Choong Kyun ^{[1
,2
]}

Rahman, Md. Aminur ^{[2
]}

机构：

[1] Chungnam Natl Univ, Dept Chem, Taejon 305764, South Korea

[2] Chungnam Natl Univ, GRAST, Taejon 305764, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 50卷

基金：

新加坡国家研究基金会;

关键词：

Electrochemical immunosensor; Non-enzymatic label; 3; 5; '-Tetramethylbenzidine; Gold nanoparticles; Polydopamine; Carcinoembryonic antigen; ELECTROCHEMICAL IMMUNOSENSOR; CANCER BIOMARKER; QUANTUM DOTS; IMMUNOASSAY; NANOPARTICLES; PROTEINS; CEA; DNA;

D O I：

10.1016/j.bios.2013.06.016

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A stable and sensitive electrochemical carcinoembryonic antigen immunosensor based on a new nonenzymatic label (3, 3', 5, 5'-tetramethylbenzidine (TMB)) was fabricated using gold nanoparticles-deposited polydopamine film (PD/AuNPs) as an anti-CEA antibody immobilizing platform. TMB was conjugated with CEA and utilized as an electrochemical label. The PD/AuNPs platform provides enhanced conductivity, thus the redox reaction of TMB label was observed at the immunosensor probe. The immunosensor probe was characterized using scanning electron microscopy and electrochemical impedance spectroscopy. The CEA detection was based on the competitive binding of free- and TMB-conjugated-CEA to the anti-CEA antibody. Using differential pulse voltammetry, CEA can be selectively detected as low as 10 +/- 0.6 pg/mL. The present immunosensor method was applied to CEA-spiked human serum samples and the recovery results were satisfactory. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：118 / 124

页数：7

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