Characterization of Escherichia coli strains with gapA and gapB genes deleted

被引:39
作者
DellaSeta, F [1 ]
BoschiMuller, S [1 ]
Vignais, ML [1 ]
Branlant, G [1 ]
机构
[1] UNIV NANCY 1,LAB ENZYMOL & GENIE GENET,URA CNRS 457,F-54506 VANDOEUVRE NANCY,FRANCE
关键词
D O I
10.1128/jb.179.16.5218-5221.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We obtained a series of Escherichia coli strains in which gapA, gapB, or both had been deleted, Delta gapA strains do not revert on glucose, while Delta gapB strains grow on glycerol or glucose, We showed that gapB-encoded protein is expressed but at a very low level, Together, these results confirm the essential role for gapA in glycolysis and show that gapB is dispensable for both glycolysis and the pyridoxal hiosynthesis pathway.
引用
收藏
页码:5218 / 5221
页数:4
相关论文
共 23 条
[1]   IDENTIFICATION, MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF A GENE-CLUSTER ENCODING THE CLASS-II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE, 3-PHOSPHOGLYCERATE KINASE AND A PUTATIVE 2ND GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE OF ESCHERICHIA-COLI [J].
ALEFOUNDER, PR ;
PERHAM, RN .
MOLECULAR MICROBIOLOGY, 1989, 3 (06) :723-732
[2]  
ALLEN RW, 1987, J BIOL CHEM, V262, P649
[3]   LINKAGE MAP OF ESCHERICHIA-COLI-K-12, EDITION-7 [J].
BACHMANN, BJ .
MICROBIOLOGICAL REVIEWS, 1983, 47 (02) :180-230
[4]   Comparative enzymatic properties of GapB-encoded erythrose-4-phosphate dehydrogenase of Escherichia coli and phosphorylating glyceraldehyde-3-phosphate dehydrogenase [J].
BoschiMuller, S ;
Azza, S ;
Pollastro, D ;
Corbier, C ;
Branlant, G .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (24) :15106-15112
[5]  
BOSCHIMULLER S, UNPUB
[6]   MOLECULAR-CLONING OF THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENES OF BACILLUS-STEAROTHERMOPHILUS AND ESCHERICHIA-COLI, AND THEIR EXPRESSION IN ESCHERICHIA-COLI [J].
BRANLANT, G ;
FLESCH, G ;
BRANLANT, C .
GENE, 1983, 25 (01) :1-7
[7]  
BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376
[8]   Identification, sequencing, and enzymatic activity of the erythrose-4-phosphate dehydrogenase gene of Vibrio cholerae [J].
Carroll, PA ;
Zhao, GS ;
Boyko, SA ;
Winkler, ME ;
Calderwood, SB .
JOURNAL OF BACTERIOLOGY, 1997, 179 (01) :293-296
[9]  
CASWELL AH, 1985, J BIOL CHEM, V260, P6892
[10]   THE ESCHERICHIA-COLI GAPA GENE IS TRANSCRIBED BY THE VEGETATIVE RNA-POLYMERASE HOLOENZYME E-SIGMA(70) AND BY THE HEAT-SHOCK RNA-POLYMERASE E-SIGMA(32) [J].
CHARPENTIER, B ;
BRANLANT, C .
JOURNAL OF BACTERIOLOGY, 1994, 176 (03) :830-839