Identification, sequencing, and enzymatic activity of the erythrose-4-phosphate dehydrogenase gene of Vibrio cholerae

被引：8

作者：

Carroll, PA

Zhao, GS

Boyko, SA

Winkler, ME

Calderwood, SB

机构：

[1] MASSACHUSETTS GEN HOSP,INFECT DIS UNIT,BOSTON,MA 02114

[2] UNIV TEXAS,SCH MED,DEPT MICROBIOL & MOL GENET,HOUSTON,TX 77030

[3] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOL GENET,BOSTON,MA 02115

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 01期

关键词：

ESCHERICHIA-COLI; TRANSCRIPTIONAL REGULATION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; NUCLEOTIDE-SEQUENCE; SUICIDE VECTOR; VIRULENCE GENE; CLONING; CONSTRUCTION; IRON; PROTEINS;

D O I：

10.1128/jb.179.1.293-296.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have identified a gene in vibrio choler ne (epd) which encodes an erythrose-4-phosphate dehydrogenase activity and is located immediately downstream of an iron-regulated virulence gene, irgA, and immediately upstream of a gene encoding phosphoglycerate kinase (pgk). Expression of epd in V. cholerae is not regulated by iron, nor is it required for virulence in an infant mouse model.

引用

收藏

页码：293 / 296

页数：4

相关论文

共 20 条

[1] IDENTIFICATION, MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF A GENE-CLUSTER ENCODING THE CLASS-II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE, 3-PHOSPHOGLYCERATE KINASE AND A PUTATIVE 2ND GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE OF ESCHERICHIA-COLI [J].

ALEFOUNDER, PR ;

PERHAM, RN .

MOLECULAR MICROBIOLOGY, 1989, 3 (06) :723-732

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] NUCLEOTIDE-SEQUENCE OF THE ESCHERICHIA-COLI GAP GENE - DIFFERENT EVOLUTIONARY BEHAVIOR OF THE NAD+-BINDING DOMAIN AND THE CATALYTIC DOMAIN OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE [J].

BRANLANT, G ;

BRANLANT, C .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 150 (01) :61-66

[4] IDENTIFICATION, CLONING, AND SEQUENCING OF A GENE REQUIRED FOR FERRIC VIBRIOBACTIN UTILIZATION BY VIBRIO-CHOLERAE [J].

BUTTERTON, JR ;

CALDERWOOD, SB .

JOURNAL OF BACTERIOLOGY, 1994, 176 (18) :5631-5638

[5] CONSTRUCTION OF AN EAE DELETION MUTANT OF ENTEROPATHOGENIC ESCHERICHIA-COLI BY USING A POSITIVE-SELECTION SUICIDE VECTOR [J].

DONNENBERG, MS ;

KAPER, JB .

INFECTION AND IMMUNITY, 1991, 59 (12) :4310-4317

[6] A NATURALLY-OCCURRING HORIZONTAL GENE-TRANSFER FROM A EUKARYOTE TO A PROKARYOTE [J].

DOOLITTLE, RF ;

FENG, DF ;

ANDERSON, KL ;

ALBERRO, MR .

JOURNAL OF MOLECULAR EVOLUTION, 1990, 31 (05) :383-388

[7] TRANSCRIPTIONAL REGULATION BY IRON OF A VIBRIO-CHOLERAE VIRULENCE GENE AND HOMOLOGY OF THE GENE TO THE ESCHERICHIA-COLI FUR SYSTEM [J].

GOLDBERG, MB ;

BOYKO, SA ;

CALDERWOOD, SB .

JOURNAL OF BACTERIOLOGY, 1990, 172 (12) :6863-6870

[8] CHARACTERIZATION OF A VIBRIO-CHOLERAE VIRULENCE FACTOR HOMOLOGOUS TO THE FAMILY OF TONB-DEPENDENT PROTEINS [J].

GOLDBERG, MB ;

BOYKO, SA ;

BUTTERTON, JR ;

STOEBNER, JA ;

PAYNE, SM ;

CALDERWOOD, SB .

MOLECULAR MICROBIOLOGY, 1992, 6 (16) :2407-2418

[9] POSITIVE TRANSCRIPTIONAL REGULATION OF AN IRON-REGULATED VIRULENCE GENE IN VIBRIO-CHOLERAE [J].

GOLDBERG, MB ;

BOYKO, SA ;

CALDERWOOD, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (04) :1125-1129

[10] IDENTIFICATION OF AN IRON-REGULATED VIRULENCE DETERMINANT IN VIBRIO-CHOLERAE, USING TNPHOA MUTAGENESIS [J].

GOLDBERG, MB ;

DIRITA, VJ ;

CALDERWOOD, SB .

INFECTION AND IMMUNITY, 1990, 58 (01) :55-60