Identification, sequencing, and enzymatic activity of the erythrose-4-phosphate dehydrogenase gene of Vibrio cholerae

被引：8

作者：

Carroll, PA

Zhao, GS

Boyko, SA

Winkler, ME

Calderwood, SB

机构：

[1] MASSACHUSETTS GEN HOSP,INFECT DIS UNIT,BOSTON,MA 02114

[2] UNIV TEXAS,SCH MED,DEPT MICROBIOL & MOL GENET,HOUSTON,TX 77030

[3] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOL GENET,BOSTON,MA 02115

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 01期

关键词：

ESCHERICHIA-COLI; TRANSCRIPTIONAL REGULATION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; NUCLEOTIDE-SEQUENCE; SUICIDE VECTOR; VIRULENCE GENE; CLONING; CONSTRUCTION; IRON; PROTEINS;

D O I：

10.1128/jb.179.1.293-296.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have identified a gene in vibrio choler ne (epd) which encodes an erythrose-4-phosphate dehydrogenase activity and is located immediately downstream of an iron-regulated virulence gene, irgA, and immediately upstream of a gene encoding phosphoglycerate kinase (pgk). Expression of epd in V. cholerae is not regulated by iron, nor is it required for virulence in an infant mouse model.

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页码：293 / 296

页数：4

相关论文

共 20 条

[11] GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MUTANTS OF ESCHERICHIA-COLI [J].

HILLMAN, JD ;

FRAENKEL, DG .

JOURNAL OF BACTERIOLOGY, 1975, 122 (03) :1175-1179

[12] ISOLATION AND CHARACTERIZATION OF ESCHERICHIA-COLI MUTANTS DEFECTIVE IN ENZYMES OF GLYCOLYSIS [J].

IRANI, M ;

MAITRA, PK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 56 (01) :127-133

[13] GLYCERALDEHYDE 3-P DEHYDROGENASE, GLYCERATE 3-P KINASE AND ENOLASE MUTANTS OF ESCHERICHIA-COLI - GENETIC STUDIES [J].

IRANI, MH ;

MAITRA, PK .

MOLECULAR & GENERAL GENETICS, 1976, 145 (01) :65-71

[14] CLONING, SEQUENCING, AND TRANSCRIPTIONAL REGULATION OF THE VIBRIO-CHOLERAE FUR GENE [J].

LITWIN, CM ;

BOYKO, SA ;

CALDERWOOD, SB .

JOURNAL OF BACTERIOLOGY, 1992, 174 (06) :1897-1903

[15] CHOLERA-TOXIN GENES - NUCLEOTIDE-SEQUENCE, DELETION ANALYSIS AND VACCINE DEVELOPMENT [J].

MEKALANOS, JJ ;

SWARTZ, DJ ;

PEARSON, GDN ;

HARFORD, N ;

GROYNE, F ;

DEWILDE, M .

NATURE, 1983, 306 (5943) :551-557

[16] A NOVEL SUICIDE VECTOR AND ITS USE IN CONSTRUCTION OF INSERTION MUTATIONS - OSMOREGULATION OF OUTER-MEMBRANE PROTEINS AND VIRULENCE DETERMINANTS IN VIBRIO-CHOLERAE REQUIRES TOXR [J].

MILLER, VL ;

MEKALANOS, JJ .

JOURNAL OF BACTERIOLOGY, 1988, 170 (06) :2575-2583

[17] EXPRESSION OF THE VIBRIO-CHOLERAE GENE ENCODING ALDEHYDE DEHYDROGENASE IS UNDER CONTROL OF TOXR, THE CHOLERA-TOXIN TRANSCRIPTIONAL ACTIVATOR [J].

PARSOT, C ;

MEKALANOS, JJ .

JOURNAL OF BACTERIOLOGY, 1991, 173 (09) :2842-2851

[18] CONSTRUCTION AND PROPERTIES OF A NEW CLONING VEHICLE, ALLOWING DIRECT SCREENING FOR RECOMBINANT PLASMIDS [J].

RUTHER, U .

MOLECULAR & GENERAL GENETICS, 1980, 178 (02) :475-477

[19] Relative importance of three iron-regulated outer membrane proteins for in vivo growth of Vibrio cholerae [J].

Tashima, KT ;

Carroll, PA ;

Rogers, MB ;

Calderwood, SB .

INFECTION AND IMMUNITY, 1996, 64 (05) :1756-1761

[20] BIOCHEMICAL-CHARACTERIZATION OF GAPB-ENCODED ERYTHROSE 4-PHOSPHATE DEHYDROGENASE OF ESCHERICHIA-COLI K-12 AND ITS POSSIBLE ROLE IN PYRIDOXAL 5'-PHOSPHATE BIOSYNTHESIS [J].

ZHAO, GS ;

PEASE, AJ ;

BHARANI, N ;

WINKLER, ME .

JOURNAL OF BACTERIOLOGY, 1995, 177 (10) :2804-2812