Soft-state biomicrofluidic pulse generator for single cell analysis

被引：31

作者：

Sabounchi, P ^{[1
]}

Ionescu-Zanetti, C ^{[1
]}

Chen, R ^{[1
]}

Karandikar, M ^{[1
]}

Seo, J ^{[1
]}

Lee, LP ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Bioengn, Berkeley Sensor & Actuator Ctr, Biomol Nanotechnol Ctr, Berkeley, CA 94720 USA

来源：

APPLIED PHYSICS LETTERS | 2006年 / 88卷 / 18期

关键词：

D O I：

10.1063/1.2195106

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We present the design, fabrication, and characterization of a soft-state biomicrofluidic pulse generator for single cell analysis. Hydrodynamic cell trapping via lateral microfluidic junctions allows the trapping of single cells from a bulk suspension. Microfluidic injection sites adjacent to the cell-trapping channels enable the pulsed delivery of nanoliter volumes of biochemical reagent. We demonstrated the application and removal of reagent at a frequency of 10 Hz with a rise time of less than 33 ms and a reagent consumption rate of 0.2 nL/s. It is shown that this system operates as a low-pass filter with a cutoff frequency of 7 Hz.

引用

页数：3