Influence of the polymeric morphology of sorbents on their properties in affinity chromatography

被引：11

作者：

Alvarez, CI ^{[1
]}

Gomez, CG ^{[1
]}

Strumia, MC ^{[1
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Quim Organ, RA-5000 Cordoba, Argentina

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2001年 / 49卷 / 1-3期

关键词：

morphology of sorbents; chemical modification of supports; Fe3+-containing sorbents; retention of phosphoamino acids; IMAC technique; macroporous polymers;

D O I：

10.1016/S0165-022X(01)00194-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of the polymeric morphology of different types of Fe3+-containing sorbents and their properties in retention of phosphoamino acids is presented in this paper. Poly(hydroxylated polybutadienic-hydroxyethyl methacrylate) [poly(PB-HEMA)] and poly(ethylene glycol dimethacrylate-hydroxyethyl methacrylate) [poly(EGDMA-HEMA)] base supports were submitted to chemical modifications to attain metal ion-containing sorbents. Properties such as specific surface area, pore volume, equilibrium volume swelling ratios, extent of conversion rate of functional groups, amount of chelated metal ion, ligand occupation, as well as quantity of phosphoamino acid retained, were used as comparative parameters for those different base matrices. Results suggest that Fe3+ immobilized on poly(EGDMA-HEMA) base support are more efficient as a group-specific sorbent to retain phosphoamino acids than those obtained using poly(PB-HEMA) base support. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：141 / 151

页数：11

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