Rapid sequence-based identification of gonococcal transmission clusters in a large metropolitan area

被引:326
作者
Martin, IMC
Ison, CA
Aanensen, DM
Fenton, KA
Spratt, BG
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis & Microbiol, London, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis Epidemiol, London, England
[3] Hlth Protect Agcy, Sexually Transmitted Bacteria Reference Lab, SRMD,HIV STI Div, Ctr Communicable Dis Surveillance, London NW9 5HT, England
[4] Royal Free & UCL, Dept Primary Care, Sch Med, London, England
[5] Royal Free & UCL, Dept Populat Sci, Sch Med, London, England
[6] Royal Free & UCL, Dept Sexually Transmitted Dis, Sch Med, London, England
来源
JOURNAL OF INFECTIOUS DISEASES | 2004年 / 189卷 / 08期
基金
英国惠康基金;
关键词
D O I
10.1086/383047
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In large metropolitan areas, which typically have the highest rates of gonorrhea, the identification of chains of transmission by use of partner notification is problematic, and there is an increasing interest in applying molecular approaches, which would require new discriminatory high-throughput procedures for recognizing clusters of indistinguishable gonococci, procedures that identify local chains of transmission. Sequencing of internal fragments of 2 highly polymorphic loci, from 436 isolates recovered in London during a 3-month period, identified clusters of antibiotic-resistant and antibiotic-susceptible isolates with indistinguishable genotypes, the vast majority of which were also identical or closely related by other methods, and defined groups of individuals who typically had similar demographic characteristics. This discriminatory sequence-based approach produces unambiguous data that easily can be compared via the Internet and appears to be suitable for the identification of linked cases of gonorrhea and the timely identification of transmission of antibiotic-resistant strains, even within large cities.
引用
收藏
页码:1497 / 1505
页数:9
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