Free solution capillary electrophoretic separation of basic proteins and drugs using cationic polymer coated capillaries

被引：17

作者：

Liu, QC ^{[1
]}

Lin, FM ^{[1
]}

Hartwick, RA ^{[1
]}

机构：

[1] PHARMASSIST ANALYT LAB INC,S NEW BERLIN,NY 13843

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1997年 / 20卷 / 05期

关键词：

D O I：

10.1080/10826079708014136

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple capillary coating which exhibits reversed (anodic) electroosmotic flow (EOF) has been developed for the separation of cationic compounds by free solution capillary electrophoresis. Poly(2-aminoethyl methacrylate hydrochloride) (PALM) was chemically bound onto the fused-silica capillary inner wall. Capillaries modified by this method exhibit an essentially pH-independent EOF in the pH range from 3 to 5.5, and have been applied to the improved separation of basic proteins and drugs (e.g., beta-adrenergic blocking drugs). High efficiency and precise migration (relative standard deviation was less than 1%) were observed.

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收藏

页码：707 / 718

页数：12

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