OASIS is a transcriptional activator of CREB/ATF family with a transmembrane domain

被引：71

作者：

Omori, Y

Imai, JI

Suzuki, Y

Watanabe, S

Tanigami, A

Sugano, S

机构：

[1] Univ Tokyo, Inst Med Sci, Ctr Human Genome, Lab Genome Struct Anal,Minato Ku, Tokyo 1088639, Japan

[2] Univ Tokyo, Inst Med Sci, Div Canc Genom, Minato Ku, Tokyo 1088639, Japan

[3] Otsuka Pharmaceut Co Ltd, Fujii Mem Res Inst, Otsu, Shiga 5200106, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

gliosis; transmembrane domain; GFAP; CREB/ATF family; SREBP; CREB-H; proteolsis; endoplasmic reticulum; membrane protein;

D O I：

10.1016/S0006-291X(02)00253-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Murine OASIS is a putative CREB/ATF family transcription factor that is induced in gliosis. but its molecular role has not been determined. We have isolated the human OASIS gene and investigated the potential of OASIS protein as a transcriptional activator. We found that OASIS can activate transcription through box-13 elements but not through the somatostatin CRE. OASIS contains a putative C-terminal hydrophobic transmembrane domain, a typical structural feature for the transcription factors activated by regulated intramembrane proteolysis. Truncation of the OASIS transmembrane domain resulted in a significant increase in transcriptional activity and altered its subcellular localization from the endoplasmic reticulum to the nucleus. Western blot analysis of transfected cells identified OASIS polypeptides of 82 and 66 kDa. These results suggest that the transmembrane domain plays an important role in the regulation of transcriptional activation by OASIS. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：470 / 477

页数：8

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