A glucose biosensor based on enzyme entrapment within polypyrrole films electrodeposited on mesoporous titanium dioxide

被引：135

作者：

Cosnier, S

Senillou, A

Grätzel, M

Comte, P

Vlachopoulos, N

Renault, NJ

Martelet, C

机构：

[1] Univ Grenoble 1, CNRS, UMR 5630, Lab Electrochim Organ & Photochim Redox, F-38041 Grenoble 9, France

[2] Ecole Polytech Fed Lausanne, Dept Chim, Lab Photon & Interfaces, CH-1015 Lausanne, Switzerland

[3] Ecole Cent Lyon, LPCI, IFOS, F-69130 Ecully, France

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1999年 / 469卷 / 02期

关键词：

biosensor; electropolymerization; glucose oxidase; polypyrrole; TiO2;

D O I：

10.1016/S0022-0728(99)00223-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrochemical immobilization of glucose oxidase (GOD) within polypyrrole films electrogenerated on mesoporous TiO2 films is described. The porosity of anatase TiO2 layers allows the diffusion of amphiphilic pyrrole ammonium tetrafluoroborate (1) and its electrochemical oxidation at the underlying SnO2 surface. The determination of glucose is carried out at - 0.15 V (vs. Ag \AgCl) via the selective reduction of H2O2 at the TiO2 surface. The analytical characteristics of different poly 1-GOD coatings have been compared. In addition, the influence of the thickness of the TiO2 layer on the performance of the biosensor for H2O2 and glucose detection has been determined. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：176 / 181

页数：6

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