CONTROLLED ELECTROCHEMICAL PREPARATION OF ENZYMATIC LAYERS FOR THE DESIGN OF AMPEROMETRIC BIOSENSORS

被引：38

作者：

COCHEGUERENTE, L

COSNIER, S

INNOCENT, C

MAILLEY, P

MOUTET, JC

MORELIS, RM

LECA, B

COULET, PR

机构：

[1] UNIV JOSEPH FOURIER,ELECTROCHIM ORGAN & PHOTOCHIM REDOX LAB,CNRS,URA 1210,BP 53X,F-38041 GRENOBLE,FRANCE

[2] LAB GENIE ENZYMAT,CNRS,F-69622 VILLEURBANNE,FRANCE

来源：

ELECTROANALYSIS | 1993年 / 5卷 / 08期

关键词：

ELECTROPOLYMERIZATION; POLYPYRROLE; AMPEROMETRIC BIOSENSORS;

D O I：

10.1002/elan.1140050804

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A newly synthesized pyrrole derivative was used for enzyme entrapment on carbon or platinum electrodes by electropolymerization. Three enzymes were tested: glucose oxidase (GOD), tyrosinase, and choline oxidase. Contrary to other techniques using electropolymerization in solution, a drop of enzyme-monomer mixture was deposited and allowed to dry on the electrode surface before the electropolymerization. This procedure enables one to control the amount of entrapped enzyme and to easily obtain multilayer assemblies.

引用

页码：647 / 652

页数：6

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