Comparison of Three Commercial Methods for Rapid Detection of Clostridium difficile Toxins A and B from Fecal Specimens

被引：77

作者：

Alcala, L. ^{[1
]}

Sanchez-Cambronero, L. ^{[1
]}

Catalan, M. P. ^{[1
]}

Sanchez-Somolinos, M. ^{[1
]}

Pelaez, M. T. ^{[1
]}

Marin, M. ^{[1
]}

Bouza, E. ^{[1
]}

机构：

[1] Univ Complutense, Hosp Gen Univ Gregorio Maranon, Microbiol Serv, Madrid 28007, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 11期

关键词：

D O I：

10.1128/JCM.01060-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three rapid enzyme immunoassays (X/pect Clostridium difficile Toxin A/B test, Wampole Tox A/B Quik Chek, and ImmunoCard Toxins A&B) were compared for the diagnosis of Clostridium difficile infection. Of the 367 stool specimens tested, 102 (27.8%) were positive for toxigenic C. difficile when a combination of direct cytotoxicity assay and cytotoxic culture was used as the gold standard. Sensitivity/specificity values were 49.0%/95.8%, 54.9%/95.5%, and 66.7%/95.1%, respectively. The median times to test five stool specimens were 28, 30, and 24 min, respectively. The ImmunoCard test was the quickest and most sensitive test of the three enzyme immunoassays evaluated.

引用

页码：3833 / 3835

页数：3

共 10 条

[1] 'Second-look' cytotoxicity:: an evaluation of culture plus cytotoxin assay of Clostridium difficile isolates in the laboratory diagnosis of CDAD [J].

Bouza, E ;

Peláez, T ;

Alonso, R ;

Catalán, P ;

Muñoz, P ;

Créixems, MR .

JOURNAL OF HOSPITAL INFECTION, 2001, 48 (03) :233-237

[2] CYTO-TOXICITY ASSAY IN ANTIBIOTIC-ASSOCIATED COLITIS [J].

CHANG, TW ;

LAUERMANN, M ;

BARTLETT, JG .

JOURNAL OF INFECTIOUS DISEASES, 1979, 140 (05) :765-770

[3]

Diederen Bram M W, 2007, J Infect, V54, pe251, DOI 10.1016/j.jinf.2007.02.008

[4] Clostridium difficile -: Associated diarrhea [J].

Johnson, S ;

Gerding, DN .

CLINICAL INFECTIOUS DISEASES, 1998, 26 (05) :1027-1034

[5] Detection of Clostridium difficile toxin:: Comparison of enzyme immunoassay results with results obtained by cytotoxicity assay [J].

Musher, Daniel M. ;

Manhas, Atisha ;

Jain, Pranav ;

Nuila, Franziska ;

Waqar, Amna ;

Logan, Nancy ;

Marino, Bernard ;

Graviss, Edward A. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (08) :2737-2739

[6] Evaluation of four immunoenzymatic tests for detecting Clostridium difficile toxins A and B [J].

Poilane, I. ;

Humeniuk-Ainouz, C. ;

Benboujida, K. ;

Kharbach, A. ;

Cruaud, P. ;

Collignon, A. .

PATHOLOGIE BIOLOGIE, 2006, 54 (8-9) :431-434

[7]

SAMRA Z, 2007, DIGEST DIS SCI, V8, P8

[8]

Sednaoui P, 1999, PATHOL BIOL, V47, P415

[9]

Toyokawa Masahiro, 2007, Kansenshogaku Zasshi, V81, P33

[10] Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients [J].

van den Berg, RJ ;

van Coppenraet, LSB ;

Gerritsen, HJ ;

Endtz, HP ;

van der Vorm, ER ;

Kuijper, EJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (10) :5338-5340

← 1 →