Human serum albumin adsorption on poly[(glycidyl methacrylate)-co-(methyl methacrylate)] beads modified with a spacer-arm-attached L-histidine ligand

被引:12
作者
Bayramoglu, G [1 ]
Senel, AÜ [1 ]
Yalçin, E [1 ]
Arica, MY [1 ]
机构
[1] Kirikkale Univ, Fac Sci, Biochem Proc & Biomat Res Lab, TR-71450 Kirikkale, Turkey
关键词
poly(GMA/MMA) beads; spacer-arm; histidine; affinity adsorbents; human serum albumin; adsorption;
D O I
10.1002/pi.1905
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Poly(GMA/MMA) beads were synthesized from glycidyl methacrylate (GMA) and methyl methacrylate (MMA) in the presence of a cross-linker (i.e. ethyleneglycol dimethacrylate) (EGDMA) via suspension polymerization. The epoxy groups of the poly(GMA/MMA) beads were converted into amino groups with either ammonia or 1,6-diaminohexane (i.e. spacer-arm). An L-histidine ligand was then covalently immobilized on the aminated (poly(GMA/MMA)-AH) and/or the spacer-arm attached (poly(GMA/MMA)-SAH) beads using glutaric dialdehyde as a coupling agent. Both affinity adsorbents were used in human serum albumin (HSA) adsorption/desorption studies under defined pH, ionic strength or temperature conditions in a batch reactor. The spacer-arm attached affinity adsorbent resulted in an increase in the adsorption capacity to HSA when compared to the aminated counterpart (i.e. poly(GMA/MMA)-AH). The maximum adsorption capacities of the affinity adsorbents were found to be significantly high, i.e. 43.7 and 80.2 mg g(-1) (of the beads), while the affinity constants, evaluated by the Langmuir model, were 3.96 x 10(-7) and 9.53 x 10(-7) molL(-1) for poly(GMA/MMA) -AH and poly(GMA/MMA)-SAH, respectively. The adsorption capacities of the affinity adsorbents were decreased for HSA by increasing the ionic strength, adjusted with NaCl. The adsorption kinetics of HSA were analysed by using pseudo-first and pseudo-second-order equations. The second-order equation fitted well with the experimental data. (c) 2005 Society of Chemical Industry.
引用
收藏
页码:40 / 48
页数:9
相关论文
共 26 条
[11]  
FESHKE KJ, 1981, BIOCHEM PHARMACOL, V30, P687
[12]   New affinity nylon membrane used for adsorption of γ-globulin [J].
Gan, HY ;
Shang, ZH ;
Wang, JD .
JOURNAL OF CHROMATOGRAPHY A, 2000, 867 (1-2) :161-168
[13]   Separation of human albumin and IgG by a membrane-based integrated bioseparation technique involving simultaneous precipitation, microfiltration and membrane adsorption [J].
Ghosh, R .
JOURNAL OF MEMBRANE SCIENCE, 2004, 237 (1-2) :109-117
[14]   ATOMIC-STRUCTURE AND CHEMISTRY OF HUMAN SERUM-ALBUMIN [J].
HE, XM ;
CARTER, DC .
NATURE, 1992, 358 (6383) :209-215
[15]   Adsorption of BSA onto sulfonated microspheres [J].
Hu, J ;
Li, SJ ;
Liu, BL .
BIOCHEMICAL ENGINEERING JOURNAL, 2005, 23 (03) :259-263
[16]   Preparation of modified hollow polypropylene membrane and their adsorption properties of γ-globulins [J].
Hwang, TS ;
Park, JW .
MACROMOLECULAR RESEARCH, 2003, 11 (05) :347-351
[17]  
KRAGHHANSEN U, 1981, PHARMACOL REV, V33, P17
[18]  
Lagergren S., 1907, K. Sven. Vetenskad. Handl, V2, DOI 10.1007/BF01501332
[19]   New packing materials for protein chromatography [J].
Leonard, M .
JOURNAL OF CHROMATOGRAPHY B, 1997, 699 (1-2) :3-27
[20]   Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) [J].
Luo, QZ ;
Zou, HF ;
Xiao, XZ ;
Guo, Z ;
Kong, L ;
Mao, XQ .
JOURNAL OF CHROMATOGRAPHY A, 2001, 926 (02) :255-264