Use of flocked swabs and a universal transport medium to enhance molecular detection of Chlamydia trachomatis and Neisseria gonorrhoeae

被引：50

作者：

Chernesky, M

Castriciano, S

Jang, D

Smieja, M

机构：

[1] McMaster Univ, St Josephs Healthcare, Hamilton, ON L8N 4A6, Canada

[2] Father Sean O Sullivan Res Ctr, Hamilton, ON L8N 4A6, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 03期

关键词：

D O I：

10.1128/JCM.44.3.1084-1086.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The use of new flocked swabs, compared to kit swabs, enhanced the ability of three commercial nucleic acid amplification tests to detect low levels of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids when the organisms were diluted in a universal transport medium as mocked specimens.

引用

收藏

页码：1084 / 1086

页数：3

相关论文

共 18 条

[1] Comparison of the APTIMA CT and GC assays with the APTIMA combo 2 assay, the Abbott LCx assay, and direct fluorescent-antibody and culture assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae [J].

Boyadzhyan, B ;

Yashina, T ;

Yatabe, JH ;

Patnaik, M ;

Hill, CS .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (07) :3089-3093

[2] Chlamydia trachomatis diagnostics [J].

Chernesky, MA .

SEXUALLY TRANSMITTED INFECTIONS, 2002, 78 (04) :232-234

[3] Specimen processing and concentration of Chlamydia trachomatis added can influence false-negative rates in the LCx assay but not in the APTIMA combo 2 assay when testing for inhibitors [J].

Chong, S ;

Jang, D ;

Song, X ;

Mahony, J ;

Petrich, A ;

Barriga, P ;

Chernesky, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (02) :778-782

[4]

Coutlée F, 2000, J CLIN MICROBIOL, V38, P2512

[5] Comparison of three nucleic acid amplification tests for detection of Chlamydia trachomatis in urine specimens [J].

Gaydos, CA ;

Theodore, M ;

Dalesio, N ;

Wood, BJ ;

Quinn, TC .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (07) :3041-3045

[6]

KELLOGG JA, 1995, AM J CLIN PATHOL, V104, P554

[7] Diff-Quik stain as a simplified alternative to Papanicolaou stain for determination of quality of endocervical specimens submitted for PCR detection of Chlamydia trachomatis [J].

Kellogg, JA ;

Seiple, JW ;

Klinedinst, JL ;

Stroll, E .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (10) :2590-2592

[8] EFFECT OF ENDOCERVICAL SPECIMEN QUALITY ON DETECTION OF CHLAMYDIA-TRACHOMATIS AND ON THE INCIDENCE OF FALSE-POSITIVE RESULTS WITH THE CHLAMYDIAZYME METHOD [J].

KELLOGG, JA ;

SEIPLE, JW ;

MURRAY, CL ;

LEVISKY, JS .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (06) :1108-1113

[9] IMPROVED PCR DETECTION OF CHLAMYDIA-TRACHOMATIS BY USING AN ALTERED METHOD OF SPECIMEN TRANSPORT AND HIGH-QUALITY ENDOCERVICAL SPECIMENS [J].

KELLOGG, JA ;

SEIPLE, JW ;

KLINEDINST, JL ;

STROLL, ES ;

CAVANAUGH, SH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (10) :2765-2767

[10] Prevalence of chlamydial and gonococcal infections among young adults in the United States [J].

Miller, WC ;

Ford, CA ;

Morris, M ;

Handcock, MS ;

Schmitz, JL ;

Hobbs, MM ;

Cohen, MS ;

Harris, KM ;

Udry, JR .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2004, 291 (18) :2229-2236