Measurement of high affinity antibodies on antigen-immunoblots

被引：14

作者：

Chapman, MD ^{[1
]}

Keir, G ^{[1
]}

Petzold, A ^{[1
]}

Thompson, EJ ^{[1
]}

机构：

[1] Inst Neurol, Dept Neuroimmunol, London WC1N 3BG, England

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2006年 / 310卷 / 1-2期

关键词：

relative antibody affinity; IEF; thiocyanate;

D O I：

10.1016/j.jim.2005.12.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a semi-quantitative method for measuring the relative affinity of antigen-specific oligoclonal IgG bands separated by isoelectric focusing followed by blotting onto antigen-coated membrane and incubation with sodium thiocyanate. When the developed blot is digitised in greyscale, densitograms can be made and peak areas calculated using lmageJ freeware. By expressing peak area as a percentage of the total area under the curve we have shown that there is a statistically significant rise in percentage of peak area for a given band which persists with increasing molarities of sodium thiocyanate. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：62 / 66

页数：5

共 19 条

[1] No evidence for production of intrathecal immunoglobulin g against Acinetobacter or Pseudomonas in multiple sclerosis [J].

Chapman, MD ;

Hughes, LE ;

Wilson, CD ;

Namnyak, S ;

Thompson, EJ ;

Giovannoni, G .

EUROPEAN NEUROLOGY, 2005, 53 (01) :27-31

[2] DETECTION AND IDENTIFICATION OF VIRUS-SPECIFIC, OLIGOCLONAL IGG IN UNCONCENTRATED CEREBROSPINAL-FLUID BY IMMUNOBLOT TECHNIQUE [J].

DORRIES, R ;

TERMEULEN, V .

JOURNAL OF NEUROIMMUNOLOGY, 1984, 7 (2-3) :77-89

[3] Antibody responses to Acinetobacter spp. and Pseudomonas aeruginosa in multiple sclerosis:: Prospects for diagnosis using the myelin-Acinetobacter-neurofilament antibody index [J].

Hughes, LE ;

Bonell, S ;

Natt, RS ;

Wilson, C ;

Tiwana, H ;

Ebringer, A ;

Cunningham, P ;

Chamoun, V ;

Thompson, EJ ;

Croker, J ;

Vowles, J .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2001, 8 (06) :1181-1188

[4] ISOELECTRIC-FOCUSING OF CEREBROSPINAL-FLUID IMMUNOGLOBULIN-G - AN ANNOTATED UPDATE [J].

KEIR, G ;

LUXTON, RW ;

THOMPSON, EJ .

ANNALS OF CLINICAL BIOCHEMISTRY, 1990, 27 :436-443

[5] DIFFERENTIAL OLIGOCLONAL BAND PATTERNS ON POLYVINYLDIFLUORIDE MEMBRANES [J].

LUXTON, RW ;

THOMPSON, EJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 121 (02) :269-274

[6] AFFINITY DISTRIBUTIONS OF ANTIGEN-SPECIFIC IGG IN PATIENTS WITH MULTIPLE-SCLEROSIS AND IN PATIENTS WITH VIRAL ENCEPHALITIS [J].

LUXTON, RW ;

THOMPSON, EJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) :277-282

[7] AFFINITY OF ANTIGEN-SPECIFIC IGG DISTINGUISHES MULTIPLE-SCLEROSIS FROM ENCEPHALITIS [J].

LUXTON, RW ;

ZEMAN, A ;

HOLZEL, H ;

HARVEY, P ;

WILSON, J ;

KOCEN, R ;

MORGANHUGHES, J ;

MILLER, DH ;

COMPSTON, A ;

THOMPSON, EJ .

JOURNAL OF THE NEUROLOGICAL SCIENCES, 1995, 132 (01) :11-19

[8] IGG PARAPROTEINS IN NEUROLOGICAL DISEASES - LACK OF ASSOCIATION WITH NEUROTROPIC VIRAL BACTERIAL-ANTIGENS [J].

MAVRA, M ;

LUXTON, R ;

THOMPSON, EJ .

ACTA NEUROLOGICA SCANDINAVICA, 1992, 86 (06) :596-598

[9]

Monteyne P, 1997, J MED VIROL, V53, P324, DOI 10.1002/(SICI)1096-9071(199712)53:4&lt

[10]

324::AID-JMV3&gt

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