Translational readthrough of the PDE2 stop codon modulates cAMP levels in Saccharomyces cerevisiae

被引：92

作者：

Namy, O

Duchateau-Nguyen, G

Rousset, JP ^{[1
]}

机构：

[1] Univ Paris 11, Inst Genet Microbiol, Lab Genet Mol Traduct, F-91405 Orsay, France

[2] Univ Paris 11, Inst Genet Microbiol, Lab Bioinformat Genomes, F-91405 Orsay, France

来源：

MOLECULAR MICROBIOLOGY | 2002年 / 43卷 / 03期

关键词：

D O I：

10.1046/j.1365-2958.2002.02770.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The efficiency of translation termination in yeast can vary several 100-fold, depending on the context around the stop codon. We performed a computer analysis designed to identify yeast open reading frames (ORFs) containing a readthrough motif surrounding the termination codon. Eight ORFs were found to display inefficient stop codon recognition, one of which, PDE2, encodes the high-affinity cAMP phosphodiesterase. We demonstrate that Pde2p stability is very impaired by the readthrough-dependent extension of the protein. A 20-fold increase in readthrough of PDE2 was observed in a [PSI+] as compared with a [psi(-)] strain. Consistent with this observation, an important increase in cAMP concentration was observed in suppressor backgrounds. These results provide a molecular explanation for at least some of the secondary phenotypes associated with suppressor backgrounds.

引用

页码：641 / 652

页数：12

共 68 条

[1] Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae [J].