Translational readthrough of the PDE2 stop codon modulates cAMP levels in Saccharomyces cerevisiae

被引:92
作者
Namy, O
Duchateau-Nguyen, G
Rousset, JP [1 ]
机构
[1] Univ Paris 11, Inst Genet Microbiol, Lab Genet Mol Traduct, F-91405 Orsay, France
[2] Univ Paris 11, Inst Genet Microbiol, Lab Bioinformat Genomes, F-91405 Orsay, France
关键词
D O I
10.1046/j.1365-2958.2002.02770.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The efficiency of translation termination in yeast can vary several 100-fold, depending on the context around the stop codon. We performed a computer analysis designed to identify yeast open reading frames (ORFs) containing a readthrough motif surrounding the termination codon. Eight ORFs were found to display inefficient stop codon recognition, one of which, PDE2, encodes the high-affinity cAMP phosphodiesterase. We demonstrate that Pde2p stability is very impaired by the readthrough-dependent extension of the protein. A 20-fold increase in readthrough of PDE2 was observed in a [PSI+] as compared with a [psi(-)] strain. Consistent with this observation, an important increase in cAMP concentration was observed in suppressor backgrounds. These results provide a molecular explanation for at least some of the secondary phenotypes associated with suppressor backgrounds.
引用
收藏
页码:641 / 652
页数:12
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