Effect of Surface Topography of Hydroxyapatite on Human Osteosarcoma MG-63 Cell

The effect of surface topography of hydroxyapatite (HA) on Human osteosarcoma MG-63 cell lines response was investigated. HA discs with various pore sizes, different pore morphology and distribution were produced by single-axis pressing method. Cell morphology and proliferation were evaluated by scanning electron microscope (SEM) and MTT methods, respectively, and the differentiation potential was assessed by alkaline phosphatase (ALP) activity testing and osteogenic gene expression (RT-PCR). The results showed that the HA discs with macroporous structure (>200 mu m) would promote cell adhesion and proliferation, while osteogenic differentiation of MG-63 cells was facilitated by the HA discs with micropore structure (<100 mu m). Moreover, the differentiation was also influenced by regularity and distribution of the pore. The irregular and alveolate micropore showed a stronger ability of osteogenic induction than smooth and shallow micropore structure.