A highly selective fluorescent probe for the detection and imaging of peroxynitrite in living cells

被引：260

作者：

Yang, Dan

Wang, Hua-Li

Sun, Zhen-Ning

Chung, Nga-Wai

Shen, Jian-Gang

机构：

[1] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

[2] Univ Hong Kong, Open Lab Chem Biol, Hong Kong, Hong Kong, Peoples R China

[3] Univ Hong Kong, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 18期

关键词：

D O I：

10.1021/ja0603756

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have found a specific reaction between ketone 1 and peroxynitrite (ONOO-), rather than other reactive oxygen species and reactive nitrogen species generated in the biological system. On the basis of this reaction, we have successfully developed a new fluorescent probe HKGreen-1, which is highly selective for the detection of peroxynitrite in living cells. Before the oxidation with peroxynitrite, the dichlorofluorescein part is masked and the probe is nonfluorescent. However, upon reaction with peroxynitrite, the fluorophore is released, resulting in strong enhancement in fluorescence intensity. Copyright © 2006 American Chemical Society.

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收藏

页码：6004 / 6005

页数：2

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